Mitotic cell cycle proteins increase in podocytes despite lack of proliferation

Arndt T. Petermann, Jeffrey Pippin, Keiju Hiromura, Toshi Monkawa, Raghu Durvasula, William G. Couser, Jeffrey Kopp, Stuart J. Shankland

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)


Background. Podocyte proliferation is an uncommon response to glomerular injury and its lack may underlie the development of glomerulosclerosis. However, whether podocytes have the capacity to enter and finish mitosis and cytokinesis is not known. Methods. The expression of mitotic cell cycle proteins (phosphorylated Histone 3, Cdc2, cyclin B1 and B2) was examined by immunohistochemistry in kidneys of embryonal mice, transgenic HIV-mice, and rats with experimental membranous nephropathy (passive Heymann nephritis, PHN). Mitotic proteins also were measured by Western blot in glomerular protein from PHN-rats and the activity of mitotic cyclins was quantified by histone kinase assay. Results. Mitotic proteins were increased in embryonal mouse glomeruli during the S- and comma-shaped stages and were absent at the capillary loop stage and in mature rodent glomeruli. There was an increase in podocyte expression of Cdc2, cyclin B1 and B2 and phosphorylated histone 3 in PHN rats, and in HIV transgenic mice. Conclusions. Podocytes have the ability to increase cell cycle proteins required for mitosis. Without obvious differences in the expression of the major mitotic proteins in PHN- and HIV-nephropathy, a regulatory disturbance in cytokinesis might be responsible for the development of polynucleated cells and a lack of podocyte proliferation in experimental glomerular disease.

Original languageEnglish
Pages (from-to)113-122
Number of pages10
JournalKidney international
Issue number1
Publication statusPublished - 2003
Externally publishedYes


  • Cdc2
  • Cell cycle
  • Cyclin B
  • Glomerular disease
  • Kidney
  • Mitosis
  • Podocyte
  • Polynucleated cells

ASJC Scopus subject areas

  • Nephrology


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