Mixed Infection of Cytomegalovirus and Pulmonary Nocardiosis Caused by Nocardia elegans Diagnosed Using Nanopore Sequencing Technology

Chie Watanabe; Yoshifumi Kimizuka; Yuji Fujikura; Takaaki Hamamoto; Akira Watanabe; Takashi Yaguchi; Tomoya Sano; Ryohei Suematsu; Yoshiki Kato; Jun Miyata; Susumu Matsukuma; Akihiko Kawana

doi:10.2169/internalmedicine.7639-21

Mixed Infection of Cytomegalovirus and Pulmonary Nocardiosis Caused by Nocardia elegans Diagnosed Using Nanopore Sequencing Technology

Chie Watanabe, Yoshifumi Kimizuka, Yuji Fujikura, Takaaki Hamamoto, Akira Watanabe, Takashi Yaguchi, Tomoya Sano, Ryohei Suematsu, Yoshiki Kato, Jun Miyata, Susumu Matsukuma, Akihiko Kawana

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A 69-year-old woman who had undergone renal transplantation and was receiving sulfamethoxazole/trimethoprim (ST) developed pulmonary nocardiosis. To our knowledge, this is the first report of the identification of Nocardia elegans using nanopore sequencing, supported by 16S rDNA capillary sequencing findings. Chest computed tomography performed after ST initiation revealed significant improvement of the pulmonary shadows compared to previous findings. We herein report the value of nanopore sequencing for rapid identification of rare pathogens, such as Nocardia elegans. Furthermore, our findings suggest that Nocardia may infect even patients receiving ST, which is currently the most effective prophylactic drug.

Original language	English
Pages (from-to)	1613-1617
Number of pages	5
Journal	Internal Medicine
Volume	61
Issue number	10
DOIs	https://doi.org/10.2169/internalmedicine.7639-21
Publication status	Published - 2022
Externally published	Yes

Keywords

immunocompromised
nanopore sequencing
nocardiosis
organ transplantation

ASJC Scopus subject areas

Internal Medicine

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10.2169/internalmedicine.7639-21

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Watanabe, C., Kimizuka, Y., Fujikura, Y., Hamamoto, T., Watanabe, A., Yaguchi, T., Sano, T., Suematsu, R., Kato, Y., Miyata, J., Matsukuma, S., & Kawana, A. (2022). Mixed Infection of Cytomegalovirus and Pulmonary Nocardiosis Caused by Nocardia elegans Diagnosed Using Nanopore Sequencing Technology. Internal Medicine, 61(10), 1613-1617. https://doi.org/10.2169/internalmedicine.7639-21

Watanabe, C, Kimizuka, Y, Fujikura, Y, Hamamoto, T, Watanabe, A, Yaguchi, T, Sano, T, Suematsu, R, Kato, Y, Miyata, J, Matsukuma, S & Kawana, A 2022, 'Mixed Infection of Cytomegalovirus and Pulmonary Nocardiosis Caused by Nocardia elegans Diagnosed Using Nanopore Sequencing Technology', Internal Medicine, vol. 61, no. 10, pp. 1613-1617. https://doi.org/10.2169/internalmedicine.7639-21

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abstract = "A 69-year-old woman who had undergone renal transplantation and was receiving sulfamethoxazole/trimethoprim (ST) developed pulmonary nocardiosis. To our knowledge, this is the first report of the identification of Nocardia elegans using nanopore sequencing, supported by 16S rDNA capillary sequencing findings. Chest computed tomography performed after ST initiation revealed significant improvement of the pulmonary shadows compared to previous findings. We herein report the value of nanopore sequencing for rapid identification of rare pathogens, such as Nocardia elegans. Furthermore, our findings suggest that Nocardia may infect even patients receiving ST, which is currently the most effective prophylactic drug.",

keywords = "immunocompromised, nanopore sequencing, nocardiosis, organ transplantation",

author = "Chie Watanabe and Yoshifumi Kimizuka and Yuji Fujikura and Takaaki Hamamoto and Akira Watanabe and Takashi Yaguchi and Tomoya Sano and Ryohei Suematsu and Yoshiki Kato and Jun Miyata and Susumu Matsukuma and Akihiko Kawana",

note = "Funding Information: This study was approved by the National Defense Medical College Review Committee. Publisher Copyright: {\textcopyright} 2022 The Japanese Society of Internal Medicine.",

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doi = "10.2169/internalmedicine.7639-21",

language = "English",

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AU - Kimizuka, Yoshifumi

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AU - Hamamoto, Takaaki

AU - Watanabe, Akira

AU - Yaguchi, Takashi

AU - Sano, Tomoya

AU - Suematsu, Ryohei

AU - Kato, Yoshiki

AU - Miyata, Jun

AU - Matsukuma, Susumu

AU - Kawana, Akihiko

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AB - A 69-year-old woman who had undergone renal transplantation and was receiving sulfamethoxazole/trimethoprim (ST) developed pulmonary nocardiosis. To our knowledge, this is the first report of the identification of Nocardia elegans using nanopore sequencing, supported by 16S rDNA capillary sequencing findings. Chest computed tomography performed after ST initiation revealed significant improvement of the pulmonary shadows compared to previous findings. We herein report the value of nanopore sequencing for rapid identification of rare pathogens, such as Nocardia elegans. Furthermore, our findings suggest that Nocardia may infect even patients receiving ST, which is currently the most effective prophylactic drug.

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Mixed Infection of Cytomegalovirus and Pulmonary Nocardiosis Caused by Nocardia elegans Diagnosed Using Nanopore Sequencing Technology

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Keywords

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