TY - JOUR
T1 - Model for paraaortic lymph node metastasis produced by orthotopic implantation of ovarian carcinoma cells in athymic nude mice
AU - Tamada, Y.
AU - Aoki, D.
AU - Nozawa, S.
AU - Irimura, T.
N1 - Funding Information:
This work was supported by grants-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (11557180, 11672162, and 12307054), the Research Association for Biotechnology, the Program for Promotion of Basic Research Activities for Innovative Biosciences and the Cosmetology Research Foundation.
PY - 2004/1
Y1 - 2004/1
N2 - Lymph node metastasis through the lymphatic vessels is a critical step in determining the outcome of ovarian cancer patients, and prognosis should be improved by preventing lymph node metastasis. However, experimental models for lymph node metastasis of ovarian carcinoma are not available. We developed an orthotopic transplantation model to study this process in nude mice using the human ovarian carcinoma cell lines, KF and MH. Highly metastatic sublines (KF-LN3 and MH-LN3) were selected in vivo in nude mice by repeated orthotopic transplantation, lymph node metastasis formation and culturing the tumour cells in vitro. Because this model seems to correspond to the advanced clinical stage of ovarian carcinomas, it should be useful in understanding the molecular biology of ovarian carcinomas and in the development of therapeutic modalities against lymph node metastasis.
AB - Lymph node metastasis through the lymphatic vessels is a critical step in determining the outcome of ovarian cancer patients, and prognosis should be improved by preventing lymph node metastasis. However, experimental models for lymph node metastasis of ovarian carcinoma are not available. We developed an orthotopic transplantation model to study this process in nude mice using the human ovarian carcinoma cell lines, KF and MH. Highly metastatic sublines (KF-LN3 and MH-LN3) were selected in vivo in nude mice by repeated orthotopic transplantation, lymph node metastasis formation and culturing the tumour cells in vitro. Because this model seems to correspond to the advanced clinical stage of ovarian carcinomas, it should be useful in understanding the molecular biology of ovarian carcinomas and in the development of therapeutic modalities against lymph node metastasis.
KW - Animal model
KW - Lymph node metastasis
KW - Ovarian cancer
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U2 - 10.1016/j.ejca.2003.08.008
DO - 10.1016/j.ejca.2003.08.008
M3 - Article
C2 - 14687800
AN - SCOPUS:0347627359
SN - 0959-8049
VL - 40
SP - 158
EP - 163
JO - European Journal of Cancer
JF - European Journal of Cancer
IS - 1
ER -
