Modification of striatal arginine and citrulline metabolism by nitric oxide synthase inhibitors

Kouichi Ohta, Kunio Shimazu, Satoru Komatsumoto, Nobuo Araki, Mamoru Shibata, Yasuo Fukuuchi

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)


The effects of NG-substituted L-arginine (ARG) analogues on striatal ARG and citrulline (CIT) levels were investigated using in vivo microdialysis technique. A microdialysis probe was implanted into the striatum of anaesthetized Sprague-Dawley rats. Direct intrastriatal perfusion with 1 mM NG-nitro-L-arginine methyl ester (n = 8) increased striatal ARG release and decreased CIT release, suggesting suppressed NO synthase activity in the tissue. On the other hand, 1 mM NG-monomethyl-L-argi-nine (L-NMMA) (n = 6) evoked a persistent increase in both ARG and CIT. Considering that 4-320 μM L-ARG (n = 8) failed to increase CIT formation, CIT seems to be synthesized in the striatal tissue from L-NMMA by the enzyme that has been demonstrated in the kidney and aortic endothelium (NG, NG-dimethylarginine dimethyl-aminohydrolase).

Original languageEnglish
Pages (from-to)766-768
Number of pages3
Issue number7
Publication statusPublished - 1994 Mar


  • Arginine
  • Citrulline
  • In vivo microdialysis
  • N
  • N-dimethylarginine dimethyl-aminohydrolase
  • N-mono-methyl-L-arginine
  • N-nitro-L-arginine methyl ester
  • Nitric oxide

ASJC Scopus subject areas

  • General Neuroscience


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