TY - JOUR
T1 - Molecular Cloning and Characterization of the HmrM Multidrug Efflux Pump from Haemophilus influenzae Rd
AU - Xu, Xing Jue
AU - Su, Xian Zhong
AU - Morita, Yuji
AU - Kuroda, Teruo
AU - Mizushima, Tohru
AU - Tsuchiya, Tomofusa
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2003
Y1 - 2003
N2 - We cloned a gene responsible for norfloxacin resistance from the chromosomal DNA of Haemophilus influenzae Rd, and designated the gene as hmrM. HmrM showed sequence similarity with NorM of Vibrio parahaemolyticus and YdhE of Escherichia coli and others that belong to the MATE family multidrug efflux pumps. The recombinant plasmid carrying the hmrM gene conferred elevated resistance not only to norfloxacin but also to acriflavine, 4′ ,6-diamidino-2-phenylindole, doxorubicin, ethidium bromide, tetraphenylphosphonium chloride, Hoechst 33342, daunomycin, berberine, and sodium deoxycholate in Escherichia coli KAM32, a drug-hypersensitive strain. We observed an Na+-dependent efflux of ethidium and an ethidium-induced efflux of Na+ in E. coli KAM32 cells harboring the plasmid carrying the hmrM gene. These results indicate that HmrM is an Na+/drug antiporter-type multidrug efflux pump. A difference in substrate preference was observed between HmrM, NorM, and YdhE.
AB - We cloned a gene responsible for norfloxacin resistance from the chromosomal DNA of Haemophilus influenzae Rd, and designated the gene as hmrM. HmrM showed sequence similarity with NorM of Vibrio parahaemolyticus and YdhE of Escherichia coli and others that belong to the MATE family multidrug efflux pumps. The recombinant plasmid carrying the hmrM gene conferred elevated resistance not only to norfloxacin but also to acriflavine, 4′ ,6-diamidino-2-phenylindole, doxorubicin, ethidium bromide, tetraphenylphosphonium chloride, Hoechst 33342, daunomycin, berberine, and sodium deoxycholate in Escherichia coli KAM32, a drug-hypersensitive strain. We observed an Na+-dependent efflux of ethidium and an ethidium-induced efflux of Na+ in E. coli KAM32 cells harboring the plasmid carrying the hmrM gene. These results indicate that HmrM is an Na+/drug antiporter-type multidrug efflux pump. A difference in substrate preference was observed between HmrM, NorM, and YdhE.
KW - Drug efflux pump
KW - Haemophilus influenzae
KW - HmrM
KW - Multidrug resistance
UR - http://www.scopus.com/inward/record.url?scp=0346734230&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0346734230&partnerID=8YFLogxK
U2 - 10.1111/j.1348-0421.2003.tb03467.x
DO - 10.1111/j.1348-0421.2003.tb03467.x
M3 - Article
C2 - 14695443
AN - SCOPUS:0346734230
SN - 0385-5600
VL - 47
SP - 937
EP - 943
JO - Microbiology and Immunology
JF - Microbiology and Immunology
IS - 12
ER -