Molecular cloning and functional characterization of a novel member of the C-C chemokine family

T. Hara, K. B. Bacon, L. C. Cho, A. Yoshimura, Y. Morikawa, N. G. Copeland, D. J. Gilbert, N. A. Jenkins, T. J. Schall, A. Miyajima

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)


Chemokines play an important role in immune and inflammatory responses by inducing migration and adhesion of leukocytes. We have isolated a novel chemokine cDNA, designated CCF18, from a cDNA library of an IL-3-dependent murine pro-B cell line, Ba/F3. The cDNA encodes a protein structurally related to the C-C chemokine members. Among this family, C10 shows the highest homology to CCF18, and MIP-1α also has a significant homology but to a lesser extent. CCF18 produced from COS cells induced chemotaxis and Ca2+ flux in CD4+ T cell clones. Moreover, prior administration of MIP-1α desensitized the cells to CCF18. The CCF18 gene (Scya10) was mapped to a middle region of murine chromosome 11, where other genes for several C-C chemokine members are localized. These results clearly indicate that CCF18 is a new member of the C-C chemokine family. Since a high level of CCF18 mRNA is constitutively expressed in macrophage and myeloid cell lines, CCF18 may play a role in inflammatory processes.

Original languageEnglish
Pages (from-to)5352-5358
Number of pages7
JournalJournal of Immunology
Issue number11
Publication statusPublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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