Molecular cloning and sequence analysis of the DnaA gene of staphylococcus aureus

Hideki Katayama; Tohru Mizushima; Takeyoshi Miki; Kazuhisa Sekimizu

doi:10.1248/bpb.20.820

Molecular cloning and sequence analysis of the DnaA gene of staphylococcus aureus

Hideki Katayama, Tohru Mizushima, Takeyoshi Miki, Kazuhisa Sekimizu

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The dnaA gene of Staphylococcus aureus has now been cloned and sequenced. The deduced amino acid sequence of Staphylococcus aureus DnaA protein is 62% and 39% identical to those of the DnaA protein from Bacillus subtilis and Escherichia coli, respectively. Expression of the dnaA gene of Staphylococcus aureus in Escherichia coli caused lethality in an oriC-dependent manner.

Original language	English
Pages (from-to)	820-822
Number of pages	3
Journal	Biological and Pharmaceutical Bulletin
Volume	20
Issue number	7
DOIs	https://doi.org/10.1248/bpb.20.820
Publication status	Published - 1997 Jan 1

Keywords

DnaA protein
Molecular cloning
Staphylococcus aureus

ASJC Scopus subject areas

Pharmacology
Pharmaceutical Science

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10.1248/bpb.20.820

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abstract = "The dnaA gene of Staphylococcus aureus has now been cloned and sequenced. The deduced amino acid sequence of Staphylococcus aureus DnaA protein is 62% and 39% identical to those of the DnaA protein from Bacillus subtilis and Escherichia coli, respectively. Expression of the dnaA gene of Staphylococcus aureus in Escherichia coli caused lethality in an oriC-dependent manner.",

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AU - Miki, Takeyoshi

AU - Sekimizu, Kazuhisa

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AB - The dnaA gene of Staphylococcus aureus has now been cloned and sequenced. The deduced amino acid sequence of Staphylococcus aureus DnaA protein is 62% and 39% identical to those of the DnaA protein from Bacillus subtilis and Escherichia coli, respectively. Expression of the dnaA gene of Staphylococcus aureus in Escherichia coli caused lethality in an oriC-dependent manner.

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KW - Staphylococcus aureus

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Molecular cloning and sequence analysis of the DnaA gene of staphylococcus aureus

Abstract

Keywords

ASJC Scopus subject areas

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