TY - JOUR
T1 - Molecular Epidemiology of Mumps Virus in Japan and Proposal of Two New Genotypes
AU - Inou, Yoko
AU - Nakayama, Tetsuo
AU - Yoshida, Naoko
AU - Uejima, Hajime
AU - Yuri, Kenji
AU - Kamada, Makoto
AU - Kumagai, Takuji
AU - Sakiyama, Hiroshi
AU - Miyata, Akiko
AU - Ochiai, Hitoshi
AU - Ihara, Toshiaki
AU - Okafuji, Teruo
AU - Okafuji, Takao
AU - Nagai, Takao
AU - Suzuki, Eitaro
AU - Shimomura, Kunihisa
AU - Ito, Yuhei
AU - Miyazaki, Chiaki
N1 - Funding Information:
This work is supported by the Na tional Natural Science Foundation of China (Grant no. 71390521, 71101069, 71301062, 71301070, 71471077, and 71271107).
PY - 2004/5
Y1 - 2004/5
N2 - We isolated 872 strains of mumps virus from naso-pharyngeal secretions in seven different districts of Japan from January 2000 to July 2001. Among them, 57 strains were geno-typed by nucleotide sequencing in part of the hemagglutinin-neuraminidase (HN) and small hydrophobic (SH) protein regions. Four different genotypes (B, G, K, and L) of mumps virus were co-circulating in Japan and the distribution of genotypes varied in geographically different districts. Two new clusters designated as genotypes K and L had more than 7% nucleotide variation in the SH gene. Among the 57 strains, 11 were classified as B, 35 as G, three as K, and eight as L, which was mainly isolated in Tokyo. We also examined 104 stains isolated in a clinic in Mie prefecture from 1993 to 2003. Genotype B was the indigenous strain and genotype K was introduced in 1994. Genotypes B and K co-circulated in the 1990s and were replaced by genotype G in 2000. There was no significant change in neutralizing test antibody titers against genotypes B, G, K, and L using seven post-vaccination sera with Hoshino strain (genotype B) and these four genotypes had a different antigenicity from genotype A. We should continue to watch on mumps virus molecular epidemiology.
AB - We isolated 872 strains of mumps virus from naso-pharyngeal secretions in seven different districts of Japan from January 2000 to July 2001. Among them, 57 strains were geno-typed by nucleotide sequencing in part of the hemagglutinin-neuraminidase (HN) and small hydrophobic (SH) protein regions. Four different genotypes (B, G, K, and L) of mumps virus were co-circulating in Japan and the distribution of genotypes varied in geographically different districts. Two new clusters designated as genotypes K and L had more than 7% nucleotide variation in the SH gene. Among the 57 strains, 11 were classified as B, 35 as G, three as K, and eight as L, which was mainly isolated in Tokyo. We also examined 104 stains isolated in a clinic in Mie prefecture from 1993 to 2003. Genotype B was the indigenous strain and genotype K was introduced in 1994. Genotypes B and K co-circulated in the 1990s and were replaced by genotype G in 2000. There was no significant change in neutralizing test antibody titers against genotypes B, G, K, and L using seven post-vaccination sera with Hoshino strain (genotype B) and these four genotypes had a different antigenicity from genotype A. We should continue to watch on mumps virus molecular epidemiology.
KW - Antigenicity
KW - Genotype
KW - Hemagglutinin-neuraminidase (HN) protein
KW - Molecular epidemiology
KW - Small hydrophobic (SH) protein
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U2 - 10.1002/jmv.20065
DO - 10.1002/jmv.20065
M3 - Article
C2 - 15042655
AN - SCOPUS:12144286619
SN - 0146-6615
VL - 73
SP - 97
EP - 104
JO - Journal of Medical Virology
JF - Journal of Medical Virology
IS - 1
ER -
