Morphogenetic capability of epithelial and mesenchymal cells dissociated from swimming starfish embryos.

M. Dan-Sohkawa; Y. Komiya; H. Kaneko; H. Yamanaka

Morphogenetic capability of epithelial and mesenchymal cells dissociated from swimming starfish embryos.

M. Dan-Sohkawa, Y. Komiya, H. Kaneko, H. Yamanaka

Research output: Contribution to journal › Article › peer-review

Original language	English
Pages (from-to)	57-60
Number of pages	4
Journal	Progress in clinical and biological research
Volume	217 A
Publication status	Published - 1986
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

Cite this

@article{61a9cdc6f8e54143903c8a2a29b5cb4c,

title = "Morphogenetic capability of epithelial and mesenchymal cells dissociated from swimming starfish embryos.",

author = "M. Dan-Sohkawa and Y. Komiya and H. Kaneko and H. Yamanaka",

note = "Funding Information: RK}~\]{ATION F£}I DIZ(\[hr~JS CIR<I~}\]~\]~<YSI N AX(~DIL IJMKS. S. Brym~, K. Fhneoka and G, Holler-l)hlan~\]re_. Developmental Biology Center, \[InJv. of (hlifornh3, IrvJne, CA 927\]7. Axolotl limb stumps ~ilh a normal anterior-i~ost-erior (AP) pattern but with dorsal-ventral (D V) confrontations between digits 2 and 3 were created by grafting bet~'een contralsteral limbs. (\]rafatn d k)st differed in ploidy. After regeneration, limbs were analyzed for skeletal and muscle imttemls and the distribution of marked ceils. Of \]2 limbs analyzed, all were normal in the AP mxJs, m~d rT~st were different in DV organization from the origin~<l stuff). The original DV discontinuities either were maintained or became resolved by shifting to a peripheral position. \]hose discontinuities in ~{~ich AV tissue confronted PD tissue were resolved in the majority of cases, whereas the discontinuities in which the confrontation was AD/PV mostly remained unresolved. Cell marker mlalysis indicated that in the majority of AD/PV confrontations, graft and host contributed roughly equally to the regenerate, whereas in the majority of AV/PD confrontations anterior tissue contrJ buted more than posteri or tissue. We conclude from these data that DV confrontations are of two types and that when confrontations are resolved cell n~vercent is involved. Further, these data lend support to the suggestion that intercalation in the limb circumference is directionally biased. Supported by NrFH grant, HD 06082. 24 POSITIONAL SPECIFICATION OF BLAST CELLS IN THE LEECH EMBRYO INVOLVES A STEPWISE CON~IITMENT. M. Shankland & D.A. Weisblat. Univ. California, Berkeley, CA 94720. The o and p bandlets of the leech embryo are parallel columns of equipotent blast cells which are committed to divergent developmental pathways as a result of their relative positions within the germinal band. The lateral or o blast cells are diverted from the P pathway to the 0 pathway by the p bandlet, and this diversion can be prevented by ablating the p bandlet soon after the cells come into contact. Later ablations reveal that the o blast cell loses its ability to switch fates and becomes committed to the O pathway in three discrete steps which affect different elements within its descendant clone. Lineage tracer injections have shown that pattern elements which display coordinate commitment arise from the same blast cell daughter, and each commitment event appears to involve an uncommitted mother cell dividing to produce one daughter which remains uncommitted, and one committed daughter whose fate no longer depends upon interaction with the p bandlet. Supported by NIH, NSF, the March of Dimes and the Rowland Foundation. Funding Information: Cells dissociated from swimming embryo of the starfish reaggregate and reconstruct the larval form, bipinnaria, via specifJc morphogenetic steps. These cells can be separated into two fractions according to the difference in the rate of reaggregation. Of the two, the quickly-aggregating fraction exhibits characteristics more closely related to those of epithelial cells, while the slowly-aggregating fraction exhibits features common with mesenchymal cells, as judged from i) affinity towards the substratum, E) serum requirements, 3) time required for the external epithelium to be established, and so on. Either of these fractions are capable of reconstructing their own characteristic bipinnaria. The role played by epithelial and mesenchymal cells in morphogenesis is discussed. This study was supported by Grant-in-Aid for Special Pro3ect Research from the MESC of Japan (Project No. 59213015). Copyright: Medline is the source for the citation and abstract of this record.",

year = "1986",

language = "English",

volume = "217 A",

pages = "57--60",

journal = "Progress in clinical and biological research",

issn = "0361-7742",

publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Morphogenetic capability of epithelial and mesenchymal cells dissociated from swimming starfish embryos.

AU - Dan-Sohkawa, M.

AU - Komiya, Y.

AU - Kaneko, H.

AU - Yamanaka, H.

N1 - Funding Information: RK}~\]{ATION F£}I DIZ(\[hr~JS CIR<I~}\]~\]~<YSI N AX(~DIL IJMKS. S. Brym~, K. Fhneoka and G, Holler-l)hlan~\]re_. Developmental Biology Center, \[InJv. of (hlifornh3, IrvJne, CA 927\]7. Axolotl limb stumps ~ilh a normal anterior-i~ost-erior (AP) pattern but with dorsal-ventral (D V) confrontations between digits 2 and 3 were created by grafting bet~'een contralsteral limbs. (\]rafatn d k)st differed in ploidy. After regeneration, limbs were analyzed for skeletal and muscle imttemls and the distribution of marked ceils. Of \]2 limbs analyzed, all were normal in the AP mxJs, m~d rT~st were different in DV organization from the origin~<l stuff). The original DV discontinuities either were maintained or became resolved by shifting to a peripheral position. \]hose discontinuities in ~{~ich AV tissue confronted PD tissue were resolved in the majority of cases, whereas the discontinuities in which the confrontation was AD/PV mostly remained unresolved. Cell marker mlalysis indicated that in the majority of AD/PV confrontations, graft and host contributed roughly equally to the regenerate, whereas in the majority of AV/PD confrontations anterior tissue contrJ buted more than posteri or tissue. We conclude from these data that DV confrontations are of two types and that when confrontations are resolved cell n~vercent is involved. Further, these data lend support to the suggestion that intercalation in the limb circumference is directionally biased. Supported by NrFH grant, HD 06082. 24 POSITIONAL SPECIFICATION OF BLAST CELLS IN THE LEECH EMBRYO INVOLVES A STEPWISE CON~IITMENT. M. Shankland & D.A. Weisblat. Univ. California, Berkeley, CA 94720. The o and p bandlets of the leech embryo are parallel columns of equipotent blast cells which are committed to divergent developmental pathways as a result of their relative positions within the germinal band. The lateral or o blast cells are diverted from the P pathway to the 0 pathway by the p bandlet, and this diversion can be prevented by ablating the p bandlet soon after the cells come into contact. Later ablations reveal that the o blast cell loses its ability to switch fates and becomes committed to the O pathway in three discrete steps which affect different elements within its descendant clone. Lineage tracer injections have shown that pattern elements which display coordinate commitment arise from the same blast cell daughter, and each commitment event appears to involve an uncommitted mother cell dividing to produce one daughter which remains uncommitted, and one committed daughter whose fate no longer depends upon interaction with the p bandlet. Supported by NIH, NSF, the March of Dimes and the Rowland Foundation. Funding Information: Cells dissociated from swimming embryo of the starfish reaggregate and reconstruct the larval form, bipinnaria, via specifJc morphogenetic steps. These cells can be separated into two fractions according to the difference in the rate of reaggregation. Of the two, the quickly-aggregating fraction exhibits characteristics more closely related to those of epithelial cells, while the slowly-aggregating fraction exhibits features common with mesenchymal cells, as judged from i) affinity towards the substratum, E) serum requirements, 3) time required for the external epithelium to be established, and so on. Either of these fractions are capable of reconstructing their own characteristic bipinnaria. The role played by epithelial and mesenchymal cells in morphogenesis is discussed. This study was supported by Grant-in-Aid for Special Pro3ect Research from the MESC of Japan (Project No. 59213015). Copyright: Medline is the source for the citation and abstract of this record.

PY - 1986

Y1 - 1986

UR - http://www.scopus.com/inward/record.url?scp=0022482607&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022482607&partnerID=8YFLogxK

M3 - Article

C2 - 3749159

AN - SCOPUS:0022482607

SN - 0361-7742

VL - 217 A

SP - 57

EP - 60

JO - Progress in clinical and biological research

JF - Progress in clinical and biological research

ER -

Morphogenetic capability of epithelial and mesenchymal cells dissociated from swimming starfish embryos.

ASJC Scopus subject areas

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