TY - JOUR
T1 - mRNA sequence of the Xenopus laevis paxillin gene and its expression
AU - Ogawa, Motoyuki
AU - Hiraoka, Yoshiki
AU - Taniguchi, Koji
AU - Sakai, Yukinao
AU - Aiso, Sadakazu
N1 - Funding Information:
This work was supported in part by a National Grant-in-Aid for the Establishment of High-Tech Research Center in a Private University from the Ministry of Education, Science, Sports and Culture, as well as Keio Gakuji Fukuzawa Memorial Funds for Advancement of Education and Research and Keio Gakuji Academic Developmental Funds from Keio University.
PY - 2001/6/28
Y1 - 2001/6/28
N2 - Paxillin is a cytoskeletal protein found in structures of focal adhesions where cells adhere to the extracellular matrix. We isolated paxillin cDNA from the Xenopus laevis ovary. The cDNA sequence encodes a protein of 539 amino acids with four LIM and five LD motifs. 80% of the amino acids of frog paxillin are shared by human and chicken paxillins. Northern analysis showed that the frog gene is expressed in the spleen, kidney, testis and ovary. Immunocytochemistry showed that paxillin protein is accumulated in the nucleus as well as in the periphery of the cytoplasm of the A6 cell. This intriguing result shows that paxillin, which has been characterized as a cytoskeletal protein, is capable of translocating to the nucleus.
AB - Paxillin is a cytoskeletal protein found in structures of focal adhesions where cells adhere to the extracellular matrix. We isolated paxillin cDNA from the Xenopus laevis ovary. The cDNA sequence encodes a protein of 539 amino acids with four LIM and five LD motifs. 80% of the amino acids of frog paxillin are shared by human and chicken paxillins. Northern analysis showed that the frog gene is expressed in the spleen, kidney, testis and ovary. Immunocytochemistry showed that paxillin protein is accumulated in the nucleus as well as in the periphery of the cytoplasm of the A6 cell. This intriguing result shows that paxillin, which has been characterized as a cytoskeletal protein, is capable of translocating to the nucleus.
KW - LIM motif
KW - Nuclear export signal
KW - Nuclear translocation
KW - Paxillin
KW - Xenopus laevis
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U2 - 10.1016/S0167-4781(01)00230-5
DO - 10.1016/S0167-4781(01)00230-5
M3 - Article
C2 - 11418191
AN - SCOPUS:0035963486
SN - 0167-4781
VL - 1519
SP - 235
EP - 240
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 3
ER -