Multifarious two-photon fluorescence microscopy employing ultrabroadband femtosecond laser pulses

Keisuke Isobe; Akira Suda; Hiroshi Hashimoto; Fumihiko Kannari; Hiroyuki Kawano; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa

doi:10.1063/1.3192289

Multifarious two-photon fluorescence microscopy employing ultrabroadband femtosecond laser pulses

Keisuke Isobe, Akira Suda, Hiroshi Hashimoto, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We demonstrate two-photon excited fluorescence (TPEF) microscopy employing the novel spectral phase modulation, which permits not only the selective excitation but also the simultaneous excitation together with controlling TPEF intensities from various fluorophores freely. The selective excitation is applied to the sequential dual-color imaging without emission crosstalk and FRET imaging of a HeLa cell. By the simultaneous excitation, dual-color imaging with various signal ratios including equal signal ratio is successfully achieved.

Original language	English
Title of host publication	Frontiers in Physics - 3rd International Meeting, IMFP 2009
Pages	43-49
Number of pages	7
DOIs	https://doi.org/10.1063/1.3192289
Publication status	Published - 2009 Nov 25
Event	3rd International Meeting on Frontiers in Physics, IMFP 2009 - Kuala Lumpur, Malaysia Duration: 2009 Jan 12 → 2009 Jan 16

Publication series

Name	AIP Conference Proceedings
Volume	1150
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Other

Other	3rd International Meeting on Frontiers in Physics, IMFP 2009
Country/Territory	Malaysia
City	Kuala Lumpur
Period	09/1/12 → 09/1/16

Keywords

Biomedical imaging
Fluorescence
Multiphoton processes

ASJC Scopus subject areas

Physics and Astronomy(all)

Access to Document

10.1063/1.3192289

Cite this

Isobe, K, Suda, A, Hashimoto, H, Kannari, F, Kawano, H, Mizuno, H, Miyawaki, A & Midorikawa, K 2009, Multifarious two-photon fluorescence microscopy employing ultrabroadband femtosecond laser pulses. in Frontiers in Physics - 3rd International Meeting, IMFP 2009. AIP Conference Proceedings, vol. 1150, pp. 43-49, 3rd International Meeting on Frontiers in Physics, IMFP 2009, Kuala Lumpur, Malaysia, 09/1/12. https://doi.org/10.1063/1.3192289

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abstract = "We demonstrate two-photon excited fluorescence (TPEF) microscopy employing the novel spectral phase modulation, which permits not only the selective excitation but also the simultaneous excitation together with controlling TPEF intensities from various fluorophores freely. The selective excitation is applied to the sequential dual-color imaging without emission crosstalk and FRET imaging of a HeLa cell. By the simultaneous excitation, dual-color imaging with various signal ratios including equal signal ratio is successfully achieved.",

keywords = "Biomedical imaging, Fluorescence, Multiphoton processes",

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AU - Isobe, Keisuke

AU - Suda, Akira

AU - Hashimoto, Hiroshi

AU - Kannari, Fumihiko

AU - Kawano, Hiroyuki

AU - Mizuno, Hideaki

AU - Miyawaki, Atsushi

AU - Midorikawa, Katsumi

PY - 2009/11/25

Y1 - 2009/11/25

N2 - We demonstrate two-photon excited fluorescence (TPEF) microscopy employing the novel spectral phase modulation, which permits not only the selective excitation but also the simultaneous excitation together with controlling TPEF intensities from various fluorophores freely. The selective excitation is applied to the sequential dual-color imaging without emission crosstalk and FRET imaging of a HeLa cell. By the simultaneous excitation, dual-color imaging with various signal ratios including equal signal ratio is successfully achieved.

AB - We demonstrate two-photon excited fluorescence (TPEF) microscopy employing the novel spectral phase modulation, which permits not only the selective excitation but also the simultaneous excitation together with controlling TPEF intensities from various fluorophores freely. The selective excitation is applied to the sequential dual-color imaging without emission crosstalk and FRET imaging of a HeLa cell. By the simultaneous excitation, dual-color imaging with various signal ratios including equal signal ratio is successfully achieved.

KW - Biomedical imaging

KW - Fluorescence

KW - Multiphoton processes

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M3 - Conference contribution

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T3 - AIP Conference Proceedings

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BT - Frontiers in Physics - 3rd International Meeting, IMFP 2009

T2 - 3rd International Meeting on Frontiers in Physics, IMFP 2009

Y2 - 12 January 2009 through 16 January 2009

ER -

Multifarious two-photon fluorescence microscopy employing ultrabroadband femtosecond laser pulses

Abstract

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Keywords

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