Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization

Hiroyuki Yoshida; Aya Nagaoka; Sachiko Nakamura; Yoshinori Sugiyama; Yasunori Okada; Shintaro Inoue

doi:10.1016/j.fob.2013.08.003

Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization

Hiroyuki Yoshida, Aya Nagaoka, Sachiko Nakamura, Yoshinori Sugiyama, Yasunori Okada, Shintaro Inoue

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Recently, we have disclosed that human KIAA1199 (hKIAA1199) is a hyaluronan (HA) binding protein implicated in HA depolymerization. Although a murine homologue (mKiaa1199) was previously cloned, no information about the function of the molecule was available. Here, we show that cells transfected with mKiaa1199 cDNA selectively catabolized HA via the clathrin-coated pit pathway. A glycosaminoglycan-binding assay demonstrated the specific binding of mKiaa1199 to HA. These results were similar to our observations with hKIAA1199, although slight differences were found in the peak sizes of the minimum degradates of HA. We conclude that like hKIAA1199, mKiaa1199 is a hyaladherin, leading to HA depolymerization.

Original language	English
Pages (from-to)	352-356
Number of pages	5
Journal	FEBS Open Bio
Volume	3
DOIs	https://doi.org/10.1016/j.fob.2013.08.003
Publication status	Published - 2013 Aug
Externally published	Yes

Keywords

Catabolism
Extracellular matrix
Hyaluronan
KIAA1199

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/j.fob.2013.08.003

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title = "Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization",

abstract = "Recently, we have disclosed that human KIAA1199 (hKIAA1199) is a hyaluronan (HA) binding protein implicated in HA depolymerization. Although a murine homologue (mKiaa1199) was previously cloned, no information about the function of the molecule was available. Here, we show that cells transfected with mKiaa1199 cDNA selectively catabolized HA via the clathrin-coated pit pathway. A glycosaminoglycan-binding assay demonstrated the specific binding of mKiaa1199 to HA. These results were similar to our observations with hKIAA1199, although slight differences were found in the peak sizes of the minimum degradates of HA. We conclude that like hKIAA1199, mKiaa1199 is a hyaladherin, leading to HA depolymerization.",

keywords = "Catabolism, Extracellular matrix, Hyaluronan, KIAA1199",

author = "Hiroyuki Yoshida and Aya Nagaoka and Sachiko Nakamura and Yoshinori Sugiyama and Yasunori Okada and Shintaro Inoue",

year = "2013",

month = aug,

doi = "10.1016/j.fob.2013.08.003",

language = "English",

volume = "3",

pages = "352--356",

journal = "FEBS Open Bio",

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T1 - Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization

AU - Yoshida, Hiroyuki

AU - Nagaoka, Aya

AU - Nakamura, Sachiko

AU - Sugiyama, Yoshinori

AU - Okada, Yasunori

AU - Inoue, Shintaro

PY - 2013/8

Y1 - 2013/8

N2 - Recently, we have disclosed that human KIAA1199 (hKIAA1199) is a hyaluronan (HA) binding protein implicated in HA depolymerization. Although a murine homologue (mKiaa1199) was previously cloned, no information about the function of the molecule was available. Here, we show that cells transfected with mKiaa1199 cDNA selectively catabolized HA via the clathrin-coated pit pathway. A glycosaminoglycan-binding assay demonstrated the specific binding of mKiaa1199 to HA. These results were similar to our observations with hKIAA1199, although slight differences were found in the peak sizes of the minimum degradates of HA. We conclude that like hKIAA1199, mKiaa1199 is a hyaladherin, leading to HA depolymerization.

AB - Recently, we have disclosed that human KIAA1199 (hKIAA1199) is a hyaluronan (HA) binding protein implicated in HA depolymerization. Although a murine homologue (mKiaa1199) was previously cloned, no information about the function of the molecule was available. Here, we show that cells transfected with mKiaa1199 cDNA selectively catabolized HA via the clathrin-coated pit pathway. A glycosaminoglycan-binding assay demonstrated the specific binding of mKiaa1199 to HA. These results were similar to our observations with hKIAA1199, although slight differences were found in the peak sizes of the minimum degradates of HA. We conclude that like hKIAA1199, mKiaa1199 is a hyaladherin, leading to HA depolymerization.

KW - Catabolism

KW - Extracellular matrix

KW - Hyaluronan

KW - KIAA1199

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JO - FEBS Open Bio

JF - FEBS Open Bio

ER -

Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization

Abstract

Keywords

ASJC Scopus subject areas

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