TY - JOUR
T1 - Mutant screening for oncogenes of Ewing’s sarcoma using yeast
AU - Kitagawa, Takao
AU - Okita, Hajime
AU - Baron, Byron
AU - Tokuda, Kazuhiro
AU - Nakamura, Mikiko
AU - Wang, Yufeng
AU - Akada, Junko
AU - Hoshida, Hisashi
AU - Akada, Rinji
AU - Kuramitsu, Yasuhiro
AU - Nakamura, Kazuyuki
N1 - Funding Information:
We thank Yukie Misumi for their technical support. The authors would like to acknowledge the utilization of the LAS-1000 and a gift of pGL4.74[hRluc/TK] vector and HEK293 cells from the DNA Core Facility of the Center for Gene Research, Yamaguchi University, supported by a grant-in-aid from the Ministry of Education, Science, Sports and Culture of Japan.
Publisher Copyright:
© 2015, Springer-Verlag Berlin Heidelberg.
PY - 2015/8/27
Y1 - 2015/8/27
N2 - Many fusion genes, which are the result of chromosomal translocation and work as an oncogene, have been recently identified, but their mode of actions is still unclear. Here, we performed a yeast mutant screening for oncogenes of Ewing’s sarcoma to easily identify essential regions responsible for fusion protein functions using a yeast genetic system. Three kinds of oncogenes including EWS/FLI1, EWS/ERG, and EWS/E1AF exhibited growth inhibition in yeast. In this screening, we identified 13 single amino acid substitution mutants which could suppress growth inhibition by oncogenes. All of the point mutation positions of the EWS/ETS family proteins were located within the ETS domain, which is responsible for the interaction with a specific DNA motif. Eight-mutated residues within the ETS domain matched to 13 completely conserved amino acid residues in the human ETS domains. Moreover, mutants also showed reduced transcriptional activities on the DKK2 promoter, which is upregulated by the EWS/ETS family, compared to that of the wild type. These results suggest that the ETS domain in the EWS/ETS family proteins may be a primary target for growth inhibition of Ewing’s sarcoma and that this yeast screening system can be applied for the functional screening of the oncogenes.
AB - Many fusion genes, which are the result of chromosomal translocation and work as an oncogene, have been recently identified, but their mode of actions is still unclear. Here, we performed a yeast mutant screening for oncogenes of Ewing’s sarcoma to easily identify essential regions responsible for fusion protein functions using a yeast genetic system. Three kinds of oncogenes including EWS/FLI1, EWS/ERG, and EWS/E1AF exhibited growth inhibition in yeast. In this screening, we identified 13 single amino acid substitution mutants which could suppress growth inhibition by oncogenes. All of the point mutation positions of the EWS/ETS family proteins were located within the ETS domain, which is responsible for the interaction with a specific DNA motif. Eight-mutated residues within the ETS domain matched to 13 completely conserved amino acid residues in the human ETS domains. Moreover, mutants also showed reduced transcriptional activities on the DKK2 promoter, which is upregulated by the EWS/ETS family, compared to that of the wild type. These results suggest that the ETS domain in the EWS/ETS family proteins may be a primary target for growth inhibition of Ewing’s sarcoma and that this yeast screening system can be applied for the functional screening of the oncogenes.
KW - ETS domain
KW - EWS/ETS family
KW - Ewing’s sarcoma
KW - Yeast
UR - http://www.scopus.com/inward/record.url?scp=84937970214&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84937970214&partnerID=8YFLogxK
U2 - 10.1007/s00253-015-6621-2
DO - 10.1007/s00253-015-6621-2
M3 - Article
C2 - 25936378
AN - SCOPUS:84937970214
SN - 0175-7598
VL - 99
SP - 6737
EP - 6744
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 16
ER -