Mutation of Y925F in focal adhesion kinase (FAK) suppresses melanoma cell proliferation and metastasis

Tomonori Kaneda; Yoshiko Sonoda; Kumi Ando; Takaharu Suzuki; Yasuhiro Sasaki; Tomoyuki Oshio; Megumi Tago; Tadashi Kasahara

doi:10.1016/j.canlet.2008.05.042

Mutation of Y925F in focal adhesion kinase (FAK) suppresses melanoma cell proliferation and metastasis

Tomonori Kaneda, Yoshiko Sonoda, Kumi Ando, Takaharu Suzuki, Yasuhiro Sasaki, Tomoyuki Oshio, Megumi Tago, Tadashi Kasahara

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

This study focused on the role of focal adhesion kinase (FAK), a cytoplasmic tyrosine kinase important for many cellular processes, in the proliferation, adhesion, and invasion of melanoma cells in vitro and in vivo. We found that the Y925F-mutation of FAK in B16F10 melanoma cells suppressed metastasis in an experimental model, which correlated well with decreased extracellular matrix dependent proliferative capability, adhesive, migrational, and invasive capabilities. Transduction of the mutation Y925F resulted in a down-regulation of the phosphorylation of Erk, the expression of VEGF, and the association of FAK with paxillin. The results provide clear evidence that 925Y of FAK is critical for melanoma metastasis and this phosphorylation site will be an anti-metastatic target.

Original language	English
Pages (from-to)	354-361
Number of pages	8
Journal	Cancer Letters
Volume	270
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2008.05.042
Publication status	Published - 2008 Nov 8

Keywords

B16F10 cells
Erk
FAK
Metastasis
Paxillin
Y925F-mutation

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2008.05.042

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title = "Mutation of Y925F in focal adhesion kinase (FAK) suppresses melanoma cell proliferation and metastasis",

abstract = "This study focused on the role of focal adhesion kinase (FAK), a cytoplasmic tyrosine kinase important for many cellular processes, in the proliferation, adhesion, and invasion of melanoma cells in vitro and in vivo. We found that the Y925F-mutation of FAK in B16F10 melanoma cells suppressed metastasis in an experimental model, which correlated well with decreased extracellular matrix dependent proliferative capability, adhesive, migrational, and invasive capabilities. Transduction of the mutation Y925F resulted in a down-regulation of the phosphorylation of Erk, the expression of VEGF, and the association of FAK with paxillin. The results provide clear evidence that 925Y of FAK is critical for melanoma metastasis and this phosphorylation site will be an anti-metastatic target.",

keywords = "B16F10 cells, Erk, FAK, Metastasis, Paxillin, Y925F-mutation",

author = "Tomonori Kaneda and Yoshiko Sonoda and Kumi Ando and Takaharu Suzuki and Yasuhiro Sasaki and Tomoyuki Oshio and Megumi Tago and Tadashi Kasahara",

note = "Funding Information: We thank Prof. Yoshikazu Sugimoto for providing the B16F10 melanoma cells. We are also grateful to Drs. Hiro Wakasugi and Yoshinori Ikarashi for technical instructions. This study was supported by a Grant-in-Aid from the High-Tech Research center Project from MEXT. ",

year = "2008",

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doi = "10.1016/j.canlet.2008.05.042",

language = "English",

volume = "270",

pages = "354--361",

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T1 - Mutation of Y925F in focal adhesion kinase (FAK) suppresses melanoma cell proliferation and metastasis

AU - Kaneda, Tomonori

AU - Sonoda, Yoshiko

AU - Ando, Kumi

AU - Suzuki, Takaharu

AU - Sasaki, Yasuhiro

AU - Oshio, Tomoyuki

AU - Tago, Megumi

AU - Kasahara, Tadashi

N1 - Funding Information: We thank Prof. Yoshikazu Sugimoto for providing the B16F10 melanoma cells. We are also grateful to Drs. Hiro Wakasugi and Yoshinori Ikarashi for technical instructions. This study was supported by a Grant-in-Aid from the High-Tech Research center Project from MEXT.

PY - 2008/11/8

Y1 - 2008/11/8

N2 - This study focused on the role of focal adhesion kinase (FAK), a cytoplasmic tyrosine kinase important for many cellular processes, in the proliferation, adhesion, and invasion of melanoma cells in vitro and in vivo. We found that the Y925F-mutation of FAK in B16F10 melanoma cells suppressed metastasis in an experimental model, which correlated well with decreased extracellular matrix dependent proliferative capability, adhesive, migrational, and invasive capabilities. Transduction of the mutation Y925F resulted in a down-regulation of the phosphorylation of Erk, the expression of VEGF, and the association of FAK with paxillin. The results provide clear evidence that 925Y of FAK is critical for melanoma metastasis and this phosphorylation site will be an anti-metastatic target.

AB - This study focused on the role of focal adhesion kinase (FAK), a cytoplasmic tyrosine kinase important for many cellular processes, in the proliferation, adhesion, and invasion of melanoma cells in vitro and in vivo. We found that the Y925F-mutation of FAK in B16F10 melanoma cells suppressed metastasis in an experimental model, which correlated well with decreased extracellular matrix dependent proliferative capability, adhesive, migrational, and invasive capabilities. Transduction of the mutation Y925F resulted in a down-regulation of the phosphorylation of Erk, the expression of VEGF, and the association of FAK with paxillin. The results provide clear evidence that 925Y of FAK is critical for melanoma metastasis and this phosphorylation site will be an anti-metastatic target.

KW - B16F10 cells

KW - Erk

KW - FAK

KW - Metastasis

KW - Paxillin

KW - Y925F-mutation

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Mutation of Y925F in focal adhesion kinase (FAK) suppresses melanoma cell proliferation and metastasis

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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