Nerve growth factor treatment induces high-potassium-evoked calcium-dependent acetylcholine release in cultured embryonic rat septal cells

Takeshi Suzuki; Masaru Kanagawa; Yuko Takada; Kazuko Fujimoto; Koichiro Kawashima

doi:10.1016/0006-8993(94)91353-6

Nerve growth factor treatment induces high-potassium-evoked calcium-dependent acetylcholine release in cultured embryonic rat septal cells

Takeshi Suzuki, Masaru Kanagawa, Yuko Takada, Kazuko Fujimoto, Koichiro Kawashima

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

The effects of dibutyryl cAMP (dbcAMP) and nerve growth factor (NGF) on acetylcholine (ACh) release from primary cultured cells of embryonic rat septum were studied. Both dbcAMP and NGF increased the activity of choline acetyltransferase and contents of intracellular and spontaneously released ACh without affecting the total protein content. High-potassium-evoked ACh release was observed in NGF-treated cells but not in untreated and dbcAMP-treated cells. These results indicate that NGF induces cholinergic maturation from induction of the ACh-synthetic enzyme to the excitation-secretion processes.

Original language	English
Pages (from-to)	311-314
Number of pages	4
Journal	Brain Research
Volume	665
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(94)91353-6
Publication status	Published - 1994 Dec 5

Keywords

Acetylcholine release
Acetylcholine synthesis
Calcium
Choline acetyltransferase
Cyclic AMP
Nerve growth factor
Septal cell

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(94)91353-6

Cite this

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title = "Nerve growth factor treatment induces high-potassium-evoked calcium-dependent acetylcholine release in cultured embryonic rat septal cells",

abstract = "The effects of dibutyryl cAMP (dbcAMP) and nerve growth factor (NGF) on acetylcholine (ACh) release from primary cultured cells of embryonic rat septum were studied. Both dbcAMP and NGF increased the activity of choline acetyltransferase and contents of intracellular and spontaneously released ACh without affecting the total protein content. High-potassium-evoked ACh release was observed in NGF-treated cells but not in untreated and dbcAMP-treated cells. These results indicate that NGF induces cholinergic maturation from induction of the ACh-synthetic enzyme to the excitation-secretion processes.",

keywords = "Acetylcholine release, Acetylcholine synthesis, Calcium, Choline acetyltransferase, Cyclic AMP, Nerve growth factor, Septal cell",

author = "Takeshi Suzuki and Masaru Kanagawa and Yuko Takada and Kazuko Fujimoto and Koichiro Kawashima",

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T1 - Nerve growth factor treatment induces high-potassium-evoked calcium-dependent acetylcholine release in cultured embryonic rat septal cells

AU - Suzuki, Takeshi

AU - Kanagawa, Masaru

AU - Takada, Yuko

AU - Fujimoto, Kazuko

AU - Kawashima, Koichiro

PY - 1994/12/5

Y1 - 1994/12/5

N2 - The effects of dibutyryl cAMP (dbcAMP) and nerve growth factor (NGF) on acetylcholine (ACh) release from primary cultured cells of embryonic rat septum were studied. Both dbcAMP and NGF increased the activity of choline acetyltransferase and contents of intracellular and spontaneously released ACh without affecting the total protein content. High-potassium-evoked ACh release was observed in NGF-treated cells but not in untreated and dbcAMP-treated cells. These results indicate that NGF induces cholinergic maturation from induction of the ACh-synthetic enzyme to the excitation-secretion processes.

AB - The effects of dibutyryl cAMP (dbcAMP) and nerve growth factor (NGF) on acetylcholine (ACh) release from primary cultured cells of embryonic rat septum were studied. Both dbcAMP and NGF increased the activity of choline acetyltransferase and contents of intracellular and spontaneously released ACh without affecting the total protein content. High-potassium-evoked ACh release was observed in NGF-treated cells but not in untreated and dbcAMP-treated cells. These results indicate that NGF induces cholinergic maturation from induction of the ACh-synthetic enzyme to the excitation-secretion processes.

KW - Acetylcholine release

KW - Acetylcholine synthesis

KW - Calcium

KW - Choline acetyltransferase

KW - Cyclic AMP

KW - Nerve growth factor

KW - Septal cell

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Nerve growth factor treatment induces high-potassium-evoked calcium-dependent acetylcholine release in cultured embryonic rat septal cells

Abstract

Keywords

ASJC Scopus subject areas

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