Neutrophil gelatinase-associated lipocalin regulates gut microbiota of mice

Katsuya Mori, Takeshi Suzuki, Shizuka Minamishima, Toru Igarashi, Kei Inoue, Daisuke Nishimura, Hiroyuki Seki, Takashige Yamada, Shizuko Kosugi, Nobuyuki Katori, Saori Hashiguchi, Hiroshi Morisaki

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Background and Aim: Because neutrophil gelatinase-associated lipocalin (NGAL) is known to provide significant bacteriostatic effects during infectious conditions, we tested the hypothesis that this protein is up-regulated and secreted into the intraluminal cavity of the gut under critically ill conditions and is thus responsible for the regulation of bacterial overgrowth. Methods: With our institutional approval, male C57BL/6J mouse (6-7weeks) were enrolled and applied for lipopolysaccharide or peritonitis model compared with naïve control. We assessed NGAL protein concentrations in intestinal lumen and up-regulation of NGAL expression in intestinal tissues in in vivo as well as ex vivo settings. Simultaneously, we examined the effects of NGAL protein administration on the growth of Escherichia coli (E.coli) in in vivo and in vitro experimental settings. The localization of NGAL in intestinal tissues and lumen was also assessed by immunohistological approach using NGAL antibody. Results: Both lipopolysaccharide and peritonitis insults evoked the marked up-regulation of NGAL mRNA and protein levels in gut tissues such as crypt cells. In addition, the administration of NGAL protein significantly inhibited the outgrowth of enteric E.coli under both in vitro and in vivo conditions, accompanied by histological evidence. Conclusion: Neutrophil gelatinase-associated lipocalin protein accompanied by apparent bacteriostatic action accumulated in the intestinal wall and streamed into the mucosal layer during critically ill state, thereby possibly shaping microbiota homeostasis in the gut.

Original languageEnglish
Pages (from-to)145-154
Number of pages10
JournalJournal of Gastroenterology and Hepatology (Australia)
Issue number1
Publication statusPublished - 2016 Jan 1


  • Bacteria
  • Gut
  • Lipocalin-2
  • Microbiota
  • Toll-like receptor

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology


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