New approaches to in vitro models of blood-brain barrier drug transport

Tetsuya Terasaki; Sumio Ohtsuki; Satoko Hori; Hitomi Takanaga; Emi Nakashima; Ken Ichi Hosoya

doi:10.1016/S1359-6446(03)02858-7

New approaches to in vitro models of blood-brain barrier drug transport

Tetsuya Terasaki, Sumio Ohtsuki, Satoko Hori, Hitomi Takanaga, Emi Nakashima, Ken Ichi Hosoya

School of Pharmacy

Research output: Contribution to journal › Review article › peer-review

150 Citations (Scopus)

Abstract

The pharmaceutical industry has been searching for an in vitro blood-brain barrier (BBB) model that preserves in vivo transporter functions in CNS drug discovery and development. The application of conditionally immortalized cell lines derived from transgenic animals harboring temperature-sensitive SV40 large T-antigen gene, is a rational and promising approach to such a workable in vitro BBB model. The established brain capillary endothelial cell lines retain the in vivo transport rate of several compounds and various forms of gene expression. Furthermore, this new approach has enabled the development of stable and reproducible co-culture models with a pericyte cell line and/or an astrocyte cell line.

Original language	English
Pages (from-to)	944-954
Number of pages	11
Journal	Drug Discovery Today
Volume	8
Issue number	20
DOIs	https://doi.org/10.1016/S1359-6446(03)02858-7
Publication status	Published - 2003 Oct 15

ASJC Scopus subject areas

Pharmacology
Drug Discovery

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AU - Terasaki, Tetsuya

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AU - Nakashima, Emi

AU - Hosoya, Ken Ichi

PY - 2003/10/15

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AB - The pharmaceutical industry has been searching for an in vitro blood-brain barrier (BBB) model that preserves in vivo transporter functions in CNS drug discovery and development. The application of conditionally immortalized cell lines derived from transgenic animals harboring temperature-sensitive SV40 large T-antigen gene, is a rational and promising approach to such a workable in vitro BBB model. The established brain capillary endothelial cell lines retain the in vivo transport rate of several compounds and various forms of gene expression. Furthermore, this new approach has enabled the development of stable and reproducible co-culture models with a pericyte cell line and/or an astrocyte cell line.

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New approaches to in vitro models of blood-brain barrier drug transport

Abstract

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