TY - JOUR
T1 - New bobtail squid (Sepiolidae: Sepiolinae) from the Ryukyu islands revealed by molecular and morphological analysis
AU - Sanchez, Gustavo
AU - Jolly, Jeffrey
AU - Reid, Amanda
AU - Sugimoto, Chikatoshi
AU - Azama, Chika
AU - Marlétaz, Ferdinand
AU - Simakov, Oleg
AU - Rokhsar, Daniel S.
N1 - Funding Information:
Funding for this project was supported by the internal funds the Okinawa Institute of Science and Technology (OIST) Molecular Genetics Unit. D.S.R. acknowledges support from the Marthella Foskett Brown Chair in Biological Sciences (UCB). We also thank staff from the OIST DNA sequencing section, Onna, Okinawa for their help with the RNA-sequencing. We thank the Miller Unit at OIST for gift of E. morsei obtained from Izu Chuo Aqua Trading Co., Ltd.; Mong-Fong Lee from the National Penghu University of Science and Technology for samples of Euprymna berryi, Euprymnasp., and Sepia-darium kochii from Taiwan; Spencer Nyholm from the University of Connecticut for samples of E. scolopes; Bret Grasse from the Marine Biological Laboratory (MBL) for his sampling and donation of S. lineolata; Satoshi Tomano for samples of S. birostrata from the Seto Inland Sea; the Fisheries Cooperative Association of Kissa in Yamaguchi Prefecture through Hisayuki Abe for samples of E. berryi; Oshima Branch, the Tokyo Metropolitan Islands Area Research and Development Center of Agriculture, Forestry and Fisheries in Tokyo Metropolis through Kunihisa Yamaguchi for samples of E. parva; the Tajima Fisheries Technology Institute in Hyogo Prefecture through Tetsuya Ohtani for samples of Sepiola birostrata; the Fisheries Cooperative Association of Hamasaka in Hyogo Prefecture through Hiroshi Miyagai for samples of Rossia sp.; the Wakkanai Fisheries Research Institute in Hokkaido Government through Minoru Sano for samples of Sepiola birostrata; Hirohito Arima from Izuohshima Diving Center for samples and help during collection of E. parva; and Ryo Minemizu from Ryo Minemizu Photo Office for samples of Euprymna sp. from Kume Island. We are indebted to the late Sydney Brenner for his support and guidance. We wish to thank Michael Kuba, Spencer Nyholm, Bethany Rader, and Bret Grasse for their helpful advice on laboratory culture of cephalopods; Jan Strugnell for sharing the RNA-seq reads of E. tasmanica; Bernhard Hausdorf from the University of Hamburg Zoologishe Institut und Zookogishes Museum for the loan of the two Eupryma bursa syntypes, and Sue Lindsay and Chao Shen at Macquarie University, Sydney for assistance with Scanning Electron Microscopy, and Shawn Miller for the helpful information about marine life around Okinawa. Special thanks also to Giambattista Bello for his insightful and detailed comments and suggestions. Finally, we also want to thank the following people for their help during the sampling at different locations in Okinawa: Aki Masunaga and Keishu Asada from OIST, Ryoko Yanagisawa from the Diving team Snack Snufkin, Thoru Miyazato from the village office of Zamami, Thoru Kikuta from the Diving shop Hero, Taihei Matayoshi from Zamami sailing, and to Prof. Kaori Wakabayashi and her student Akari Yoshimoto from Hiroshima University.
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Bobtail squid are emerging models for host–microbe interactions, behavior, and development, yet their species diversity and distribution remain poorly characterized. Here, we combine mitochondrial and transcriptome sequences with morphological analysis to describe three species of bobtail squid (Sepiolidae: Sepiolinae) from the Ryukyu archipelago, and compare them with related taxa. One Ryukyuan type was previously unknown, and is described here as Euprymna brenneri sp. nov. Another Ryukyuan type is morphologically indistinguishable from Sepiola parva Sasaki, 1913. Molecular analyses, however, place this taxon within the genus Euprymna Steenstrup, 1887, and additional morphological investigation led to formal rediagnosis of Euprymna and reassignment of this species as Euprymna parva comb. nov. While no adults from the third Ryukyuan type were found, sequences from hatchlings suggest a close relationship with E. pardalota Reid, 2011, known from Australia and East Timor. The broadly sampled transcriptomes reported here provide a foundation for future phylogenetic and comparative studies.
AB - Bobtail squid are emerging models for host–microbe interactions, behavior, and development, yet their species diversity and distribution remain poorly characterized. Here, we combine mitochondrial and transcriptome sequences with morphological analysis to describe three species of bobtail squid (Sepiolidae: Sepiolinae) from the Ryukyu archipelago, and compare them with related taxa. One Ryukyuan type was previously unknown, and is described here as Euprymna brenneri sp. nov. Another Ryukyuan type is morphologically indistinguishable from Sepiola parva Sasaki, 1913. Molecular analyses, however, place this taxon within the genus Euprymna Steenstrup, 1887, and additional morphological investigation led to formal rediagnosis of Euprymna and reassignment of this species as Euprymna parva comb. nov. While no adults from the third Ryukyuan type were found, sequences from hatchlings suggest a close relationship with E. pardalota Reid, 2011, known from Australia and East Timor. The broadly sampled transcriptomes reported here provide a foundation for future phylogenetic and comparative studies.
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U2 - 10.1038/s42003-019-0661-6
DO - 10.1038/s42003-019-0661-6
M3 - Article
C2 - 31840110
AN - SCOPUS:85077582245
SN - 2399-3642
VL - 2
JO - Communications biology
JF - Communications biology
IS - 1
M1 - 465
ER -