New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies

Toshio Morizane; Itsurou Satoh; Naoki Kumagai; Hidetsugu Saito; Akiko Iino; Yasutaka Inagaki; Tetsu Watanabe; Kanji Tsuchimoto; Masaharu Tsuchiya

doi:10.1016/0022-1759(86)90482-5

New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies

Toshio Morizane, Itsurou Satoh, Naoki Kumagai, Hidetsugu Saito, Akiko Iino, Yasutaka Inagaki, Tetsu Watanabe, Kanji Tsuchimoto, Masaharu Tsuchiya

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A new micro-glass-tube leukocyte adherence inhibition (LAI) assay which is appropriate for detecting delayed type hypersensitivity in vitro has been developed for human leukocytes. Enumeration of adherent cells is replaced by a cellular radioimmunoassay determining antibody binding of the monoclonal reagents, OKT4, OKT8, and OKM1, to glass-adherent cells, fixed by glutaraldehyde or formaldehyde. An LAI reactivity to purified protein derivative of tuberculin (PPD) was detectable in donors giving a positive PPD skin test with OKT4 reagent, but not with the other two reagents.

Original language	English
Pages (from-to)	225-229
Number of pages	5
Journal	Journal of Immunological Methods
Volume	91
Issue number	2
DOIs	https://doi.org/10.1016/0022-1759(86)90482-5
Publication status	Published - 1986 Jul 24
Externally published	Yes

Keywords

Delayed-type hypersensitivity
Leukocyte adherence inhibition
Purified protein derivative of tuberculin

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0022-1759(86)90482-5

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Morizane, T., Satoh, I., Kumagai, N., Saito, H., Iino, A., Inagaki, Y., Watanabe, T., Tsuchimoto, K., & Tsuchiya, M. (1986). New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies. Journal of Immunological Methods, 91(2), 225-229. https://doi.org/10.1016/0022-1759(86)90482-5

Morizane, T, Satoh, I, Kumagai, N, Saito, H, Iino, A, Inagaki, Y, Watanabe, T, Tsuchimoto, K & Tsuchiya, M 1986, 'New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies', Journal of Immunological Methods, vol. 91, no. 2, pp. 225-229. https://doi.org/10.1016/0022-1759(86)90482-5

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title = "New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies",

abstract = "A new micro-glass-tube leukocyte adherence inhibition (LAI) assay which is appropriate for detecting delayed type hypersensitivity in vitro has been developed for human leukocytes. Enumeration of adherent cells is replaced by a cellular radioimmunoassay determining antibody binding of the monoclonal reagents, OKT4, OKT8, and OKM1, to glass-adherent cells, fixed by glutaraldehyde or formaldehyde. An LAI reactivity to purified protein derivative of tuberculin (PPD) was detectable in donors giving a positive PPD skin test with OKT4 reagent, but not with the other two reagents.",

keywords = "Delayed-type hypersensitivity, Leukocyte adherence inhibition, Purified protein derivative of tuberculin",

author = "Toshio Morizane and Itsurou Satoh and Naoki Kumagai and Hidetsugu Saito and Akiko Iino and Yasutaka Inagaki and Tetsu Watanabe and Kanji Tsuchimoto and Masaharu Tsuchiya",

note = "Funding Information: This study was supported by a grant from the Ministry of Education, Science and Culture of Japan and by a Grant for the Study of Hepatoma from IBM Japan, Ltd.",

year = "1986",

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doi = "10.1016/0022-1759(86)90482-5",

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T1 - New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies

AU - Morizane, Toshio

AU - Satoh, Itsurou

AU - Kumagai, Naoki

AU - Saito, Hidetsugu

AU - Iino, Akiko

AU - Inagaki, Yasutaka

AU - Watanabe, Tetsu

AU - Tsuchimoto, Kanji

AU - Tsuchiya, Masaharu

N1 - Funding Information: This study was supported by a grant from the Ministry of Education, Science and Culture of Japan and by a Grant for the Study of Hepatoma from IBM Japan, Ltd.

PY - 1986/7/24

Y1 - 1986/7/24

N2 - A new micro-glass-tube leukocyte adherence inhibition (LAI) assay which is appropriate for detecting delayed type hypersensitivity in vitro has been developed for human leukocytes. Enumeration of adherent cells is replaced by a cellular radioimmunoassay determining antibody binding of the monoclonal reagents, OKT4, OKT8, and OKM1, to glass-adherent cells, fixed by glutaraldehyde or formaldehyde. An LAI reactivity to purified protein derivative of tuberculin (PPD) was detectable in donors giving a positive PPD skin test with OKT4 reagent, but not with the other two reagents.

AB - A new micro-glass-tube leukocyte adherence inhibition (LAI) assay which is appropriate for detecting delayed type hypersensitivity in vitro has been developed for human leukocytes. Enumeration of adherent cells is replaced by a cellular radioimmunoassay determining antibody binding of the monoclonal reagents, OKT4, OKT8, and OKM1, to glass-adherent cells, fixed by glutaraldehyde or formaldehyde. An LAI reactivity to purified protein derivative of tuberculin (PPD) was detectable in donors giving a positive PPD skin test with OKT4 reagent, but not with the other two reagents.

KW - Delayed-type hypersensitivity

KW - Leukocyte adherence inhibition

KW - Purified protein derivative of tuberculin

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New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies

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Keywords

ASJC Scopus subject areas

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