New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies

Toshio Morizane; Itsurou Satoh; Naoki Kumagai; Hidetsugu Saito; Akiko Iino; Yasutaka Inagaki; Tetsu Watanabe; Kanji Tsuchimoto; Masaharu Tsuchiya

doi:10.1016/0022-1759(86)90482-5

New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies

Toshio Morizane
, Itsurou Satoh
, Naoki Kumagai
, Hidetsugu Saito
, Akiko Iino
, Yasutaka Inagaki
, Tetsu Watanabe
, Kanji Tsuchimoto
, Masaharu Tsuchiya

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A new micro-glass-tube leukocyte adherence inhibition (LAI) assay which is appropriate for detecting delayed type hypersensitivity in vitro has been developed for human leukocytes. Enumeration of adherent cells is replaced by a cellular radioimmunoassay determining antibody binding of the monoclonal reagents, OKT4, OKT8, and OKM1, to glass-adherent cells, fixed by glutaraldehyde or formaldehyde. An LAI reactivity to purified protein derivative of tuberculin (PPD) was detectable in donors giving a positive PPD skin test with OKT4 reagent, but not with the other two reagents.

Original language	English
Pages (from-to)	225-229
Number of pages	5
Journal	Journal of Immunological Methods
Volume	91
Issue number	2
DOIs	https://doi.org/10.1016/0022-1759(86)90482-5
Publication status	Published - 1986 Jul 24
Externally published	Yes

Keywords

Delayed-type hypersensitivity
Leukocyte adherence inhibition
Purified protein derivative of tuberculin

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0022-1759(86)90482-5

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Morizane, T., Satoh, I., Kumagai, N., Saito, H., Iino, A., Inagaki, Y., Watanabe, T., Tsuchimoto, K., & Tsuchiya, M. (1986). New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies. Journal of Immunological Methods, 91(2), 225-229. https://doi.org/10.1016/0022-1759(86)90482-5

Morizane, T, Satoh, I, Kumagai, N, Saito, H, Iino, A, Inagaki, Y, Watanabe, T, Tsuchimoto, K & Tsuchiya, M 1986, 'New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies', Journal of Immunological Methods, vol. 91, no. 2, pp. 225-229. https://doi.org/10.1016/0022-1759(86)90482-5

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title = "New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies",

abstract = "A new micro-glass-tube leukocyte adherence inhibition (LAI) assay which is appropriate for detecting delayed type hypersensitivity in vitro has been developed for human leukocytes. Enumeration of adherent cells is replaced by a cellular radioimmunoassay determining antibody binding of the monoclonal reagents, OKT4, OKT8, and OKM1, to glass-adherent cells, fixed by glutaraldehyde or formaldehyde. An LAI reactivity to purified protein derivative of tuberculin (PPD) was detectable in donors giving a positive PPD skin test with OKT4 reagent, but not with the other two reagents.",

keywords = "Delayed-type hypersensitivity, Leukocyte adherence inhibition, Purified protein derivative of tuberculin",

author = "Toshio Morizane and Itsurou Satoh and Naoki Kumagai and Hidetsugu Saito and Akiko Iino and Yasutaka Inagaki and Tetsu Watanabe and Kanji Tsuchimoto and Masaharu Tsuchiya",

note = "Funding Information: This study was supported by a grant from the Ministry of Education, Science and Culture of Japan and by a Grant for the Study of Hepatoma from IBM Japan, Ltd.",

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doi = "10.1016/0022-1759(86)90482-5",

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T1 - New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies

AU - Morizane, Toshio

AU - Satoh, Itsurou

AU - Kumagai, Naoki

AU - Saito, Hidetsugu

AU - Iino, Akiko

AU - Inagaki, Yasutaka

AU - Watanabe, Tetsu

AU - Tsuchimoto, Kanji

AU - Tsuchiya, Masaharu

N1 - Funding Information: This study was supported by a grant from the Ministry of Education, Science and Culture of Japan and by a Grant for the Study of Hepatoma from IBM Japan, Ltd.

PY - 1986/7/24

Y1 - 1986/7/24

N2 - A new micro-glass-tube leukocyte adherence inhibition (LAI) assay which is appropriate for detecting delayed type hypersensitivity in vitro has been developed for human leukocytes. Enumeration of adherent cells is replaced by a cellular radioimmunoassay determining antibody binding of the monoclonal reagents, OKT4, OKT8, and OKM1, to glass-adherent cells, fixed by glutaraldehyde or formaldehyde. An LAI reactivity to purified protein derivative of tuberculin (PPD) was detectable in donors giving a positive PPD skin test with OKT4 reagent, but not with the other two reagents.

AB - A new micro-glass-tube leukocyte adherence inhibition (LAI) assay which is appropriate for detecting delayed type hypersensitivity in vitro has been developed for human leukocytes. Enumeration of adherent cells is replaced by a cellular radioimmunoassay determining antibody binding of the monoclonal reagents, OKT4, OKT8, and OKM1, to glass-adherent cells, fixed by glutaraldehyde or formaldehyde. An LAI reactivity to purified protein derivative of tuberculin (PPD) was detectable in donors giving a positive PPD skin test with OKT4 reagent, but not with the other two reagents.

KW - Delayed-type hypersensitivity

KW - Leukocyte adherence inhibition

KW - Purified protein derivative of tuberculin

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New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies

Abstract

Keywords

ASJC Scopus subject areas

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