TY - JOUR
T1 - Nitrogen-doped carbon dots/Ni-MnFe-layered double hydroxides (N-CDs/Ni-MnFe-LDHs) hybrid nanomaterials as immunoassay label for low-density lipoprotein detection
AU - Prakobkij, Akarapong
AU - Jarujamrus, Purim
AU - Chunta, Suticha
AU - Chawengkirttikul, Runglawan
AU - Keawin, Tinnagon
AU - Malahom, Nutthaporn
AU - Tamuang, Suparb
AU - Amatathongchai, Maliwan
AU - Citterio, Daniel
N1 - Funding Information:
P. J. and A. P. would like to acknowledge the research grants from the National Research Council of Thailand (NRCT5-RSA63023-01 and NRCT5-RGJ63020, respectively) and IRN/502/2563 chaired by D. N. The Center of Excellence for Innovation in Chemistry (PERCH-CIC), Ministry of Higher Education, Science, Research, and Innovation is gratefully acknowledged. The research scholarships and instrumental facilities of the Department of Chemistry, Faculty of Science, Ubon Ratchathani University, and Department of Microbiology, Faculty of Science, Mahidol University, are also gratefully acknowledged.
Funding Information:
P. J. and A. P. would like to acknowledge the research grants from the National Research Council of Thailand (NRCT5-RSA63023-01 and NRCT5-RGJ63020, respectively) and IRN/502/2563 chaired by D. N. The Center of Excellence for Innovation in Chemistry (PERCH-CIC), Ministry of Higher Education, Science, Research, and Innovation is gratefully acknowledged. The research scholarships and instrumental facilities of the Department of Chemistry, Faculty of Science, Ubon Ratchathani University, and Department of Microbiology, Faculty of Science, Mahidol University, are also gratefully acknowledged.
Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
PY - 2022/2
Y1 - 2022/2
N2 - Nitrogen-doped carbon dots/Ni-MnFe-layered double hydroxides (N-CDs/Ni-MnFe-LDHs) are demonstrated as superior peroxidase mimic antibody labels alternative to horseradish peroxidase (HRP) in an immunoassay, potentially overcoming some of the inherent disadvantages of HRP and other enzyme mimicking nanomaterials. They revealed efficient peroxidase-like activity and catalyzed the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to form the intense blue product (at 620 nm) in the presence of hydrogen peroxide (H2O2). Using low-density lipoprotein (LDL) as a model target, an ultra-low limit of detection (0.0051 mg/dL) and a linear range of 0.0625–0.750 mg/dL were achieved, exhibiting higher sensitivity than the HRP-based immunoassay. Thus, the proposed N-CDs/Ni-MnFe-LDHs can be used as HRP mimicking analogs for developing highly sensitive colorimetric immunosensors for detection of biomarkers, as well as trace chemical analysis. Graphical abstract: [Figure not available: see fulltext.]
AB - Nitrogen-doped carbon dots/Ni-MnFe-layered double hydroxides (N-CDs/Ni-MnFe-LDHs) are demonstrated as superior peroxidase mimic antibody labels alternative to horseradish peroxidase (HRP) in an immunoassay, potentially overcoming some of the inherent disadvantages of HRP and other enzyme mimicking nanomaterials. They revealed efficient peroxidase-like activity and catalyzed the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to form the intense blue product (at 620 nm) in the presence of hydrogen peroxide (H2O2). Using low-density lipoprotein (LDL) as a model target, an ultra-low limit of detection (0.0051 mg/dL) and a linear range of 0.0625–0.750 mg/dL were achieved, exhibiting higher sensitivity than the HRP-based immunoassay. Thus, the proposed N-CDs/Ni-MnFe-LDHs can be used as HRP mimicking analogs for developing highly sensitive colorimetric immunosensors for detection of biomarkers, as well as trace chemical analysis. Graphical abstract: [Figure not available: see fulltext.]
KW - Enzyme mimic
KW - Immunoassay label
KW - Low-density lipoprotein
KW - Ni-MnFe-layered double hydroxides
KW - Nitrogen-doped carbon dots
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U2 - 10.1007/s00604-022-05173-0
DO - 10.1007/s00604-022-05173-0
M3 - Article
C2 - 35075569
AN - SCOPUS:85123751917
SN - 0026-3672
VL - 189
JO - Mikrochimica Acta
JF - Mikrochimica Acta
IS - 2
M1 - 72
ER -