NO/cGMP/PKG signaling pathway induces magnesium release mediated by mitoKATP channel opening in rat hippocampal neurons

Ryu Yamanaka; Yutaka Shindo; Kohji Hotta; Koji Suzuki; Kotaro Oka

doi:10.1016/j.febslet.2013.06.049

NO/cGMP/PKG signaling pathway induces magnesium release mediated by mitoK_ATP channel opening in rat hippocampal neurons

Ryu Yamanaka, Yutaka Shindo, Kohji Hotta, Koji Suzuki, Kotaro Oka

Department of Biosciences and Informatics

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Intracellular Mg²⁺ concentration ([_Mg2+]i) and NO regulate cell survival and death. To reveal the involvement of NO in intracellular Mg²⁺ regulation, we visualized intracellular Mg ²⁺ using the fluorescent Mg²⁺ indicator KMG-104-AM in rat hippocampal neurons. Pharmacological experiments using SNAP, 8-Br-cGMP, diazoxide and several inhibitors revealed that the NO/cGMP/Protein kinsase G (PKG) signaling pathway triggers an increase in [_Mg2+]i, and that Mg²⁺ mobilization is due to Mg²⁺ release from mitochondria induced by mitoK_ATP channel opening. In addition, Mg²⁺ release is potentiated by the positive feedback loop including mitoK_ATP channel opening, mitochondrial depolarization and PKC activation.

Original language	English
Pages (from-to)	2643-2648
Number of pages	6
Journal	FEBS Letters
Volume	587
Issue number	16
DOIs	https://doi.org/10.1016/j.febslet.2013.06.049
Publication status	Published - 2013 Aug 19

Keywords

Hippocampal neuron
KMG-104 mitoK channel
Mg
Mitochondria

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2013.06.049

Cite this

@article{41659330b76b444490434cc93e175375,

title = "NO/cGMP/PKG signaling pathway induces magnesium release mediated by mitoKATP channel opening in rat hippocampal neurons",

abstract = "Intracellular Mg2+ concentration ([Mg2+]i) and NO regulate cell survival and death. To reveal the involvement of NO in intracellular Mg2+ regulation, we visualized intracellular Mg 2+ using the fluorescent Mg2+ indicator KMG-104-AM in rat hippocampal neurons. Pharmacological experiments using SNAP, 8-Br-cGMP, diazoxide and several inhibitors revealed that the NO/cGMP/Protein kinsase G (PKG) signaling pathway triggers an increase in [Mg2+]i, and that Mg2+ mobilization is due to Mg2+ release from mitochondria induced by mitoKATP channel opening. In addition, Mg2+ release is potentiated by the positive feedback loop including mitoKATP channel opening, mitochondrial depolarization and PKC activation.",

keywords = "Hippocampal neuron, KMG-104 mitoK channel, Mg, Mitochondria",

author = "Ryu Yamanaka and Yutaka Shindo and Kohji Hotta and Koji Suzuki and Kotaro Oka",

note = "Funding Information: This work is supported by Grants-in-Aid for Scientific Research (A) ( 24240045 ), and Strategic Research Foundation Grant-aided Project for Private Universities from Ministry of Education , Culture, Sport, Science, and Technology, Japan (MEXT) , 2008–2012, ( S0801008 ).",

year = "2013",

month = aug,

day = "19",

doi = "10.1016/j.febslet.2013.06.049",

language = "English",

volume = "587",

pages = "2643--2648",

journal = "FEBS Letters",

issn = "0014-5793",

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T1 - NO/cGMP/PKG signaling pathway induces magnesium release mediated by mitoKATP channel opening in rat hippocampal neurons

AU - Yamanaka, Ryu

AU - Shindo, Yutaka

AU - Hotta, Kohji

AU - Suzuki, Koji

AU - Oka, Kotaro

N1 - Funding Information: This work is supported by Grants-in-Aid for Scientific Research (A) ( 24240045 ), and Strategic Research Foundation Grant-aided Project for Private Universities from Ministry of Education , Culture, Sport, Science, and Technology, Japan (MEXT) , 2008–2012, ( S0801008 ).

PY - 2013/8/19

Y1 - 2013/8/19

N2 - Intracellular Mg2+ concentration ([Mg2+]i) and NO regulate cell survival and death. To reveal the involvement of NO in intracellular Mg2+ regulation, we visualized intracellular Mg 2+ using the fluorescent Mg2+ indicator KMG-104-AM in rat hippocampal neurons. Pharmacological experiments using SNAP, 8-Br-cGMP, diazoxide and several inhibitors revealed that the NO/cGMP/Protein kinsase G (PKG) signaling pathway triggers an increase in [Mg2+]i, and that Mg2+ mobilization is due to Mg2+ release from mitochondria induced by mitoKATP channel opening. In addition, Mg2+ release is potentiated by the positive feedback loop including mitoKATP channel opening, mitochondrial depolarization and PKC activation.

AB - Intracellular Mg2+ concentration ([Mg2+]i) and NO regulate cell survival and death. To reveal the involvement of NO in intracellular Mg2+ regulation, we visualized intracellular Mg 2+ using the fluorescent Mg2+ indicator KMG-104-AM in rat hippocampal neurons. Pharmacological experiments using SNAP, 8-Br-cGMP, diazoxide and several inhibitors revealed that the NO/cGMP/Protein kinsase G (PKG) signaling pathway triggers an increase in [Mg2+]i, and that Mg2+ mobilization is due to Mg2+ release from mitochondria induced by mitoKATP channel opening. In addition, Mg2+ release is potentiated by the positive feedback loop including mitoKATP channel opening, mitochondrial depolarization and PKC activation.

KW - Hippocampal neuron

KW - KMG-104 mitoK channel

KW - Mg

KW - Mitochondria

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