TY - JOUR
T1 - Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog
AU - Yoshimatsu, Sho
AU - Edamura, Kazuya
AU - Yoshii, Yumi
AU - Iguchi, Aozora
AU - Kondo, Hirotaka
AU - Shibuya, Hisashi
AU - Sato, Tsukika
AU - Shiozawa, Seiji
AU - Okano, Hideyuki
N1 - Funding Information:
We especially thank Dr. Takashi Sasaki (Keio University) for technical support for the transcriptomic analysis, Ms. Kanae Ohtsu (Keio University) for assisting MEF and reagent preparation, Mr. Yuki Iwata (Nihon University) for assisting the teratoma analysis, and Maebashi Institute of. Animal Science (Gunma, Japan) for assisting the STR analysis. We also thank all the laboratory members of H.O. and K.E. for their encouragement and generous support. Data shown in this study were obtained in the “Construction of System for Spread of Primate Model Animals” project under the Strategic Research Program for Brain Sciences and Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) of the MEXT and the AMED (Grant ID: JP20dm0207001 to H.O.). This study was also supported by JSPS KAKENHI Grant Number 19J12871 and 20K22660 (to S.Y), and 20H03156 (to K.E.) and internal budgets from Keio University including the Program for the Advancement of Research in Core Projects on Longevity of the Keio University Global Research Institute from Keio University (to H.O.).
Funding Information:
We especially thank Dr. Takashi Sasaki (Keio University) for technical support for the transcriptomic analysis, Ms. Kanae Ohtsu (Keio University) for assisting MEF and reagent preparation, Mr. Yuki Iwata (Nihon University) for assisting the teratoma analysis, and Maebashi Institute of. Animal Science (Gunma, Japan) for assisting the STR analysis. We also thank all the laboratory members of H.O. and K.E. for their encouragement and generous support. Data shown in this study were obtained in the “Construction of System for Spread of Primate Model Animals” project under the Strategic Research Program for Brain Sciences and Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) of the MEXT and the AMED (Grant ID: JP20dm0207001 to H.O.). This study was also supported by JSPS KAKENHI Grant Number 19J12871 and 20K22660 (to S.Y), and 20H03156 (to K.E.) and internal budgets from Keio University including the Program for the Advancement of Research in Core Projects on Longevity of the Keio University Global Research Institute from Keio University (to H.O.).
Publisher Copyright:
© 2021 The Author(s)
PY - 2021/5
Y1 - 2021/5
N2 - We previously reported the non-viral derivation of transgene-free induced pluripotent stem cells (iPSCs) from somatic fibroblasts of a female beagle dog using an optimized induction medium and integration-free episomal vectors. Here, we report novel derivation of a male canine iPSC line OF35Y-iPS, which showed standard characteristics of pluripotency such as a strong gene expression profile of pluripotency markers, differentiation potential into all three germ layers, and normal karyotype (78XY). Furthermore, we demonstrated targeted integration of 2A-EGFP into the canine NANOS3 locus. The novel iPSC line would be a useful resource for stem cell research and regenerative veterinary medicine.
AB - We previously reported the non-viral derivation of transgene-free induced pluripotent stem cells (iPSCs) from somatic fibroblasts of a female beagle dog using an optimized induction medium and integration-free episomal vectors. Here, we report novel derivation of a male canine iPSC line OF35Y-iPS, which showed standard characteristics of pluripotency such as a strong gene expression profile of pluripotency markers, differentiation potential into all three germ layers, and normal karyotype (78XY). Furthermore, we demonstrated targeted integration of 2A-EGFP into the canine NANOS3 locus. The novel iPSC line would be a useful resource for stem cell research and regenerative veterinary medicine.
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U2 - 10.1016/j.scr.2021.102375
DO - 10.1016/j.scr.2021.102375
M3 - Article
C2 - 34088004
AN - SCOPUS:85104930686
SN - 1873-5061
VL - 53
JO - Stem Cell Research
JF - Stem Cell Research
M1 - 102375
ER -