Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF)

Terence G. Henares; Yuta Uenoyama; Yuto Nogawa; Ken Ikegami; Daniel Citterio; Koji Suzuki; Shun Ichi Funano; Kenji Sueyoshi; Tatsuro Endo; Hideaki Hisamoto

doi:10.1039/c3an00098b

Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF)

Terence G. Henares, Yuta Uenoyama, Yuto Nogawa, Ken Ikegami, Daniel Citterio, Koji Suzuki, Shun Ichi Funano, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

This paper presents a novel rhodamine diphosphate molecule that allows highly sensitive detection of proteins by employing sequential enzyme-linked immunosorbent assay and capillary isoelectric focusing (ELISA-cIEF). Seven-fold improvement in the immunoassay sensitivity and a 1-2 order of magnitude lower detection limit has been demonstrated by taking advantage of the combination of the enzyme-based signal amplification of ELISA and the concentration of enzyme reaction products by cIEF.

Original language	English
Pages (from-to)	3139-3141
Number of pages	3
Journal	Analyst
Volume	138
Issue number	11
DOIs	https://doi.org/10.1039/c3an00098b
Publication status	Published - 2013 Jun 7

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

Access to Document

10.1039/c3an00098b

Cite this

@article{2ab001cbe3ea4e2a8792fc9b3e22d35c,

title = "Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF)",

abstract = "This paper presents a novel rhodamine diphosphate molecule that allows highly sensitive detection of proteins by employing sequential enzyme-linked immunosorbent assay and capillary isoelectric focusing (ELISA-cIEF). Seven-fold improvement in the immunoassay sensitivity and a 1-2 order of magnitude lower detection limit has been demonstrated by taking advantage of the combination of the enzyme-based signal amplification of ELISA and the concentration of enzyme reaction products by cIEF.",

author = "Henares, {Terence G.} and Yuta Uenoyama and Yuto Nogawa and Ken Ikegami and Daniel Citterio and Koji Suzuki and Funano, {Shun Ichi} and Kenji Sueyoshi and Tatsuro Endo and Hideaki Hisamoto",

year = "2013",

month = jun,

day = "7",

doi = "10.1039/c3an00098b",

language = "English",

volume = "138",

pages = "3139--3141",

journal = "Analyst",

issn = "0003-2654",

publisher = "Royal Society of Chemistry",

number = "11",

}

TY - JOUR

T1 - Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF)

AU - Henares, Terence G.

AU - Uenoyama, Yuta

AU - Nogawa, Yuto

AU - Ikegami, Ken

AU - Citterio, Daniel

AU - Suzuki, Koji

AU - Funano, Shun Ichi

AU - Sueyoshi, Kenji

AU - Endo, Tatsuro

AU - Hisamoto, Hideaki

PY - 2013/6/7

Y1 - 2013/6/7

N2 - This paper presents a novel rhodamine diphosphate molecule that allows highly sensitive detection of proteins by employing sequential enzyme-linked immunosorbent assay and capillary isoelectric focusing (ELISA-cIEF). Seven-fold improvement in the immunoassay sensitivity and a 1-2 order of magnitude lower detection limit has been demonstrated by taking advantage of the combination of the enzyme-based signal amplification of ELISA and the concentration of enzyme reaction products by cIEF.

AB - This paper presents a novel rhodamine diphosphate molecule that allows highly sensitive detection of proteins by employing sequential enzyme-linked immunosorbent assay and capillary isoelectric focusing (ELISA-cIEF). Seven-fold improvement in the immunoassay sensitivity and a 1-2 order of magnitude lower detection limit has been demonstrated by taking advantage of the combination of the enzyme-based signal amplification of ELISA and the concentration of enzyme reaction products by cIEF.

UR - http://www.scopus.com/inward/record.url?scp=84877652860&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877652860&partnerID=8YFLogxK

U2 - 10.1039/c3an00098b

DO - 10.1039/c3an00098b

M3 - Article

C2 - 23596627

AN - SCOPUS:84877652860

SN - 0003-2654

VL - 138

SP - 3139

EP - 3141

JO - Analyst

JF - Analyst

IS - 11

ER -

Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF)

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this