Novel screening method for agents that overcome classical multidrug resistance in a human cell line

A. Yoshimura; N. Shudo; S. I. Ikeda; M. Ichikawa; T. Sumizawa; S. I. Akiyama

doi:10.1016/0304-3835(90)90177-Y

Novel screening method for agents that overcome classical multidrug resistance in a human cell line

A. Yoshimura, N. Shudo, S. I. Ikeda, M. Ichikawa, T. Sumizawa, S. I. Akiyama

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

P-glycoprotein (P-gp) is involved in the transport of a wide variety of organic compounds including a fluorescent dye, rhodamine 6G (RG), as well as anti-cancer drugs. Agents that overcome classical multidrugresistance (MDR) increased the accumulation of RG in an MDR cell line, KB-C1. The effect of agents on RG-accumulation in KB-C1 cells was highly correlated with their effect on the reversion of vincristine-resistance in KB-C1 cells. RG was detected on a fluorescence microplate reader with a rhodamine channel. This assay may become a useful method for the screening of agents that overcome classical MBR, since it is quick and simple.

Original language	English
Pages (from-to)	45-51
Number of pages	7
Journal	Cancer Letters
Volume	50
Issue number	1
DOIs	https://doi.org/10.1016/0304-3835(90)90177-Y
Publication status	Published - 1990 Apr 9
Externally published	Yes

Keywords

KB cell line
fluorescent dye
multidrug-resistance
resistance modifiers
screening method

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0304-3835(90)90177-Y

Cite this

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abstract = "P-glycoprotein (P-gp) is involved in the transport of a wide variety of organic compounds including a fluorescent dye, rhodamine 6G (RG), as well as anti-cancer drugs. Agents that overcome classical multidrugresistance (MDR) increased the accumulation of RG in an MDR cell line, KB-C1. The effect of agents on RG-accumulation in KB-C1 cells was highly correlated with their effect on the reversion of vincristine-resistance in KB-C1 cells. RG was detected on a fluorescence microplate reader with a rhodamine channel. This assay may become a useful method for the screening of agents that overcome classical MBR, since it is quick and simple.",

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AU - Yoshimura, A.

AU - Shudo, N.

AU - Ikeda, S. I.

AU - Ichikawa, M.

AU - Sumizawa, T.

AU - Akiyama, S. I.

PY - 1990/4/9

Y1 - 1990/4/9

N2 - P-glycoprotein (P-gp) is involved in the transport of a wide variety of organic compounds including a fluorescent dye, rhodamine 6G (RG), as well as anti-cancer drugs. Agents that overcome classical multidrugresistance (MDR) increased the accumulation of RG in an MDR cell line, KB-C1. The effect of agents on RG-accumulation in KB-C1 cells was highly correlated with their effect on the reversion of vincristine-resistance in KB-C1 cells. RG was detected on a fluorescence microplate reader with a rhodamine channel. This assay may become a useful method for the screening of agents that overcome classical MBR, since it is quick and simple.

AB - P-glycoprotein (P-gp) is involved in the transport of a wide variety of organic compounds including a fluorescent dye, rhodamine 6G (RG), as well as anti-cancer drugs. Agents that overcome classical multidrugresistance (MDR) increased the accumulation of RG in an MDR cell line, KB-C1. The effect of agents on RG-accumulation in KB-C1 cells was highly correlated with their effect on the reversion of vincristine-resistance in KB-C1 cells. RG was detected on a fluorescence microplate reader with a rhodamine channel. This assay may become a useful method for the screening of agents that overcome classical MBR, since it is quick and simple.

KW - KB cell line

KW - fluorescent dye

KW - multidrug-resistance

KW - resistance modifiers

KW - screening method

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Novel screening method for agents that overcome classical multidrug resistance in a human cell line

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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