Abstract
P-glycoprotein (P-gp) is involved in the transport of a wide variety of organic compounds including a fluorescent dye, rhodamine 6G (RG), as well as anti-cancer drugs. Agents that overcome classical multidrugresistance (MDR) increased the accumulation of RG in an MDR cell line, KB-C1. The effect of agents on RG-accumulation in KB-C1 cells was highly correlated with their effect on the reversion of vincristine-resistance in KB-C1 cells. RG was detected on a fluorescence microplate reader with a rhodamine channel. This assay may become a useful method for the screening of agents that overcome classical MBR, since it is quick and simple.
| Original language | English |
|---|---|
| Pages (from-to) | 45-51 |
| Number of pages | 7 |
| Journal | Cancer Letters |
| Volume | 50 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1990 Apr 9 |
| Externally published | Yes |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- KB cell line
- fluorescent dye
- multidrug-resistance
- resistance modifiers
- screening method
ASJC Scopus subject areas
- Oncology
- Cancer Research
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