Nuclear transfer to study the nuclear reprogramming of human stem cells

Shigeo Saito, Ken Sawai, Yoshinobu Murayama, Keiichi Fukuda, Kazunari Yokoyama

Research output: Chapter in Book/Report/Conference proceedingChapter


Research of stem cells will enable us to understand the development and function of tissues and organs in mammals. The ability to induce regeneration of new tissues from embryonic stem (ES) cells derived from cloned blastocysts via nuclear transfer can be expected in the not-too-distant future. The fact that there is no way except nuclear cloning for the return of differentiated cells to undifferentiated cells remains an interesting problem to be solved. We describe protocols for the production of cloned calves from bovine ES cells to study nuclear reprogramming ability of stem cells. The frequency of term pregnancies for blastocysts from ES cells is higher than those of early pregnancies and maintained pregnancies after nuclear transfer with bovine somatic cells. We also describe protocols for gene introduction into bovine ES cells in vitro, particularly the human leukocyte antigens (HLA). Bovine ES cells provide a powerful tool for the generation of transgenic clonal offspring. This technique, when perfected for humans, may be critical for neural stem cell transplantation.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Number of pages19
Publication statusPublished - 2008
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)10643745


  • Bovine
  • Cloning
  • Differentiation
  • Donor
  • EGF
  • EGFP
  • ES cell
  • Fusion
  • HLA
  • LIF
  • Microsatellite
  • Nuclear transfer
  • Oct-4
  • PCR
  • Pluripotency
  • Recipient
  • Reprogramming
  • STAT-3
  • Stem cells
  • Transfection

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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