TY - JOUR
T1 - Observation and quantitative analysis of rat bone marrow stromal cells cultured in vitro on newly formed transparent β-tricalcium phosphate
AU - Kotobuki, Noriko
AU - Kawagoe, Daisuke
AU - Nomura, Daishirou
AU - Katou, Youichi
AU - Muraki, Kaori
AU - Fujimori, Hirotaka
AU - Goto, Seishi
AU - Ioku, Koji
AU - Ohgushi, Hajime
PY - 2006/1/1
Y1 - 2006/1/1
N2 - To observe living cell morphology on ceramics by light microscopy, we fabricated a new material - transparent β-tricalcium phosphate (t-βTCP) ceramic - for the purpose of serving as a tissue culture substrate. Bone marrow stromal cells (BMSCs) were obtained from rat femora and cultured on both t-βTCP ceramic disks and culture grade polystyrene (PS) dishes in an osteogenic medium. After 1 day of culture, cell attachment and spreading on both the t-βTCP and PS substrata were equally and clearly detected by ordinary light microscopy. After 14 days of culture, extensive cell growth, alkaline phosphatase (ALP) staining, and bone mineral deposition could be detected on both substrata. In addition, quantitative biochemical analyses revealed high DNA content, ALP activity, and osteocalcin content of these cultures. This experiment is significant in that all of the results were similarly observed on both the t-βTCP and PS substrata, indicating the excellent properties of βTCP ceramics for BMSCs culture towards osteogenic differentiation.
AB - To observe living cell morphology on ceramics by light microscopy, we fabricated a new material - transparent β-tricalcium phosphate (t-βTCP) ceramic - for the purpose of serving as a tissue culture substrate. Bone marrow stromal cells (BMSCs) were obtained from rat femora and cultured on both t-βTCP ceramic disks and culture grade polystyrene (PS) dishes in an osteogenic medium. After 1 day of culture, cell attachment and spreading on both the t-βTCP and PS substrata were equally and clearly detected by ordinary light microscopy. After 14 days of culture, extensive cell growth, alkaline phosphatase (ALP) staining, and bone mineral deposition could be detected on both substrata. In addition, quantitative biochemical analyses revealed high DNA content, ALP activity, and osteocalcin content of these cultures. This experiment is significant in that all of the results were similarly observed on both the t-βTCP and PS substrata, indicating the excellent properties of βTCP ceramics for BMSCs culture towards osteogenic differentiation.
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U2 - 10.1007/s10856-006-6327-1
DO - 10.1007/s10856-006-6327-1
M3 - Article
C2 - 16389470
AN - SCOPUS:29944435435
SN - 0957-4530
VL - 17
SP - 33
EP - 41
JO - Journal of Materials Science: Materials in Medicine
JF - Journal of Materials Science: Materials in Medicine
IS - 1
ER -