TY - JOUR
T1 - Omentopexy enhances graft function in myocardial cell sheet transplantation
AU - Suzuki, Ryo
AU - Hattori, Fumiyuki
AU - Itabashi, Yuji
AU - Yoshioka, Masatoyo
AU - Yuasa, Shinsuke
AU - Manabe-Kawaguchi, Haruko
AU - Murata, Mitsushige
AU - Makino, Shinji
AU - Kokaji, Kiyokazu
AU - Yozu, Ryohei
AU - Fukuda, Keiichi
N1 - Funding Information:
This study was supported in part by research grants from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation (NIBIO).
PY - 2009/9/18
Y1 - 2009/9/18
N2 - Myocardial cell sheets (MCS) are a potentially valuable tool for tissue engineering aimed at heart regeneration. Several methods have recently been established for the fabrication of MCS. However, the lack of a sufficient blood supply has inhibited functional recovery of the MCS. To address this challenge, we combined MCS transplantation with omentopexy (OP), which utilizes omental tissue as a surgical flap. Rats were divided into five groups: sham, myocardial infarction (MI), MCS transplantation, OP, and MCS + OP. Histologic analysis revealed that MCS + OP drastically reversed MI-induced cardiac remodeling. Echocardiography revealed that MCS increased cardiac function, while OP had a synergistic beneficial effect with MCS transplantation. Immunofluorescence imaging showed that OP increased the survival of transplanted cardiomyocytes, and increased the blood supply through enhancement of angiogenesis and migration of small arteries into the MCS. Taken together, we concluded that OP is a promising strategy for the enhancement of graft function in MCS transplantation.
AB - Myocardial cell sheets (MCS) are a potentially valuable tool for tissue engineering aimed at heart regeneration. Several methods have recently been established for the fabrication of MCS. However, the lack of a sufficient blood supply has inhibited functional recovery of the MCS. To address this challenge, we combined MCS transplantation with omentopexy (OP), which utilizes omental tissue as a surgical flap. Rats were divided into five groups: sham, myocardial infarction (MI), MCS transplantation, OP, and MCS + OP. Histologic analysis revealed that MCS + OP drastically reversed MI-induced cardiac remodeling. Echocardiography revealed that MCS increased cardiac function, while OP had a synergistic beneficial effect with MCS transplantation. Immunofluorescence imaging showed that OP increased the survival of transplanted cardiomyocytes, and increased the blood supply through enhancement of angiogenesis and migration of small arteries into the MCS. Taken together, we concluded that OP is a promising strategy for the enhancement of graft function in MCS transplantation.
KW - Infarct
KW - Myocardial cell sheet
KW - Omentopexy
KW - Regenerative medicine
KW - Transplant
UR - http://www.scopus.com/inward/record.url?scp=67949095731&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67949095731&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2009.07.024
DO - 10.1016/j.bbrc.2009.07.024
M3 - Article
C2 - 19595673
AN - SCOPUS:67949095731
SN - 0006-291X
VL - 387
SP - 353
EP - 359
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -