One-step synthesis of n-octyl β-D-xylotrioside, xylobioside and xyloside from xylan and n-octanol using acetone powder of Aureobasidium pullulans in supercritical fluids

n-Octyl β-D-xylotrioside, xylobioside and xyloside, which are effective next-generation surfactants, can be prepared by the one-step reaction of xylan and n-octanol using the acetone powder (acetone-dried cells) of Aureobasidium pullulans as the enzyme source of xylanase in supercritical carbon dioxide (CO₂) and fluoroform (CHF₃) fluids. Yields of the n-octyl glycosides were significantly increased using supercritical CHF₃, and the total yields of the n-octyl β-D-glycosides exceeded 250 mg g^-1 xylan, n-Octyl β-D-xylotrioside was produced only by using supercritical fluids.