Optical observation of DNA motion during and immediately after nanopore translocation

Hirohito Yamazaki, Shintaro Ito, Keiko Esashika, Toshiharu Saiki

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Herein, we report an optical nanopore detection system capable of investigating DNA motion near nanopores not only during translocation but also post-translocation with submillisecond resolution. Using our optical nanopore detection system, we observed the voltage dependence of the dwell time of both 10-kbp double-stranded DNA (dsDNA) and lambda DNA in the excitation volume, which can be attributed to the drift-dominated motion. We found that the lambda DNA had slower drift motion than 10-kbp dsDNA, indicating that DNA with longer gyration experiences a lower nonuniform electric force.

Original languageEnglish
Article number017001
JournalApplied Physics Express
Volume9
Issue number1
DOIs
Publication statusPublished - 2016 Jan

ASJC Scopus subject areas

  • General Engineering
  • General Physics and Astronomy

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