TY - JOUR
T1 - Orexins increase mRNA expressions of neurotrophin-3 in rat primary cortical neuron cultures
AU - Yamada, Nobuko
AU - Katsuura, Goro
AU - Tatsuno, Ichiro
AU - Kawahara, Shigenori
AU - Ebihara, Ken
AU - Saito, Yasushi
AU - Nakao, Kazuwa
PY - 2009/1/30
Y1 - 2009/1/30
N2 - Orexins and melanin-concentrating hormone (MCH) as orexigenic neuropeptides are present in the lateral hypothalamus, and their receptors are distributed in the cerebral cortex and hippocampus. In the present study, the regulatory effects of orexin-A, orexin-B and MCH on neurotrophin-3 (NT-3) and brain-derived neurotrophic factor (BDNF) expressions were examined in primary cortical neuron cultures using quantitative real-time PCR. Both orexin-A and orexin-B on 6-day exposure significantly increased the NT-3 mRNA at concentrations of 0.01, 0.1 and 1 μM. Orexin-A and B at 1 μM led to an increase of twofold or more over the control. However, no such NT-s mRNA increase occurred with exposure to MCH at the same concentrations as orexins. The mRNA expression of BDNF was significantly increased only by orexin-B at 1 μM. These findings suggest that orexins, but not MCH, may be an inducer of NT-3 in the cerebral cortex.
AB - Orexins and melanin-concentrating hormone (MCH) as orexigenic neuropeptides are present in the lateral hypothalamus, and their receptors are distributed in the cerebral cortex and hippocampus. In the present study, the regulatory effects of orexin-A, orexin-B and MCH on neurotrophin-3 (NT-3) and brain-derived neurotrophic factor (BDNF) expressions were examined in primary cortical neuron cultures using quantitative real-time PCR. Both orexin-A and orexin-B on 6-day exposure significantly increased the NT-3 mRNA at concentrations of 0.01, 0.1 and 1 μM. Orexin-A and B at 1 μM led to an increase of twofold or more over the control. However, no such NT-s mRNA increase occurred with exposure to MCH at the same concentrations as orexins. The mRNA expression of BDNF was significantly increased only by orexin-B at 1 μM. These findings suggest that orexins, but not MCH, may be an inducer of NT-3 in the cerebral cortex.
KW - Brain-derived neurotrophic factor
KW - Melanin-concentrating hormone
KW - Neurotrophin-3
KW - Orexin
KW - Rat primary cortical neuron cultures
KW - Real-time PCR
UR - http://www.scopus.com/inward/record.url?scp=58149347086&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149347086&partnerID=8YFLogxK
U2 - 10.1016/j.neulet.2008.11.028
DO - 10.1016/j.neulet.2008.11.028
M3 - Article
C2 - 19026718
AN - SCOPUS:58149347086
SN - 0304-3940
VL - 450
SP - 132
EP - 135
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 2
ER -