TY - JOUR
T1 - Paper-Based Antibody Detection Devices Using Bioluminescent BRET-Switching Sensor Proteins
AU - Tenda, Keisuke
AU - van Gerven, Benice
AU - Arts, Remco
AU - Hiruta, Yuki
AU - Merkx, Maarten
AU - Citterio, Daniel
N1 - Funding Information:
This project has been partially supported by a Japan Society for the Promotion of Science (JSPS) Bilateral Joint Research Projects/Seminars grant and by a Grant-in-aid for Scientific Research (B) (Grant No. 18H02008) from JSPS to D.C. as well as by an ERC Starting Grant (ERC-2011-StG 280255) and ERC proof of concept grants (ERC-2013-PoC 632274 and ERC-2016-PoC 755471) to M.M.
Publisher Copyright:
© 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.
PY - 2018/11/19
Y1 - 2018/11/19
N2 - This work reports on fully integrated “sample-in-signal-out” microfluidic paper-based analytical devices (μPADs) relying on bioluminescence resonance energy transfer (BRET) switches for analyte recognition and colorimetric signal generation. The devices use BRET-based antibody sensing proteins integrated into vertically assembled layers of functionalized paper, and their design enables sample volume-independent and fully reagent-free operation, including on-device blood plasma separation. User operation is limited to the application of a single drop (20–30 μL) of sample (serum, whole blood) and the acquisition of a photograph 20 min after sample introduction, with no requirement for precise pipetting, liquid handling, or analytical equipment except for a camera. Simultaneous detection of three different antibodies (anti-HIV1, anti-HA, and anti-DEN1) in whole blood was achieved. Given its simplicity, this type of device is ideally suited for user-friendly point-of-care testing in low-resource environments.
AB - This work reports on fully integrated “sample-in-signal-out” microfluidic paper-based analytical devices (μPADs) relying on bioluminescence resonance energy transfer (BRET) switches for analyte recognition and colorimetric signal generation. The devices use BRET-based antibody sensing proteins integrated into vertically assembled layers of functionalized paper, and their design enables sample volume-independent and fully reagent-free operation, including on-device blood plasma separation. User operation is limited to the application of a single drop (20–30 μL) of sample (serum, whole blood) and the acquisition of a photograph 20 min after sample introduction, with no requirement for precise pipetting, liquid handling, or analytical equipment except for a camera. Simultaneous detection of three different antibodies (anti-HIV1, anti-HA, and anti-DEN1) in whole blood was achieved. Given its simplicity, this type of device is ideally suited for user-friendly point-of-care testing in low-resource environments.
KW - antibodies
KW - bioluminescence
KW - paper-based analytical devices
KW - point-of-care testing
KW - whole-blood analysis
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U2 - 10.1002/anie.201808070
DO - 10.1002/anie.201808070
M3 - Article
C2 - 30168634
AN - SCOPUS:85053519434
SN - 1433-7851
VL - 57
SP - 15369
EP - 15373
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 47
ER -