Paradoxical ATP elevation in ischemic penumbra revealed by quantitative imaging mass spectrometry

Katsuji Hattori; Mayumi Kajimura; Takako Hishiki; Tsuyoshi Nakanishi; Akiko Kubo; Yoshiko Nagahata; Mitsuyo Ohmura; Ayako Yachie-Kinoshita; Tomomi Matsuura; Takayuki Morikawa; Tomomi Nakamura; Mitsutoshi Setou; Makoto Suematsu

doi:10.1089/ars.2010.3290

Paradoxical ATP elevation in ischemic penumbra revealed by quantitative imaging mass spectrometry

Katsuji Hattori, Mayumi Kajimura, Takako Hishiki, Tsuyoshi Nakanishi, Akiko Kubo, Yoshiko Nagahata, Mitsuyo Ohmura, Ayako Yachie-Kinoshita, Tomomi Matsuura, Takayuki Morikawa, Tomomi Nakamura, Mitsutoshi Setou, Makoto Suematsu

Research output: Contribution to journal › Review article › peer-review

82 Citations (Scopus)

Abstract

Local responses of energy metabolism during brain ischemia are too heterogeneous to decipher redox distribution between anoxic core and adjacent salvageable regions such as penumbra. Imaging mass spectrometry combined by capillary electrophoresis/mass spectrometry providing quantitative metabolomics revealed spatio-temporal changes in adenylates and NADH in a mouse middle-cerebral artery occlusion model. Unlike the core where ATP decreased, the penumbra displayed paradoxical elevation of ATP despite the constrained blood supply. It is noteworthy that the NADH elevation in the ischemic region is clearly demarcated by the ATP-depleting core. Results suggest that metabolism in ischemic penumbra does not respond passively to compromised circulation, but actively compensates energy charges.

Original language	English
Pages (from-to)	1157-1167
Number of pages	11
Journal	Antioxidants and Redox Signaling
Volume	13
Issue number	8
DOIs	https://doi.org/10.1089/ars.2010.3290
Publication status	Published - 2010 Oct 15

ASJC Scopus subject areas

Biochemistry
Physiology
Molecular Biology
Clinical Biochemistry
Cell Biology

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Hattori, K., Kajimura, M., Hishiki, T., Nakanishi, T., Kubo, A., Nagahata, Y., Ohmura, M., Yachie-Kinoshita, A., Matsuura, T., Morikawa, T., Nakamura, T., Setou, M., & Suematsu, M. (2010). Paradoxical ATP elevation in ischemic penumbra revealed by quantitative imaging mass spectrometry. Antioxidants and Redox Signaling, 13(8), 1157-1167. https://doi.org/10.1089/ars.2010.3290

Hattori, K, Kajimura, M, Hishiki, T, Nakanishi, T, Kubo, A, Nagahata, Y, Ohmura, M, Yachie-Kinoshita, A, Matsuura, T, Morikawa, T, Nakamura, T, Setou, M & Suematsu, M 2010, 'Paradoxical ATP elevation in ischemic penumbra revealed by quantitative imaging mass spectrometry', Antioxidants and Redox Signaling, vol. 13, no. 8, pp. 1157-1167. https://doi.org/10.1089/ars.2010.3290

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abstract = "Local responses of energy metabolism during brain ischemia are too heterogeneous to decipher redox distribution between anoxic core and adjacent salvageable regions such as penumbra. Imaging mass spectrometry combined by capillary electrophoresis/mass spectrometry providing quantitative metabolomics revealed spatio-temporal changes in adenylates and NADH in a mouse middle-cerebral artery occlusion model. Unlike the core where ATP decreased, the penumbra displayed paradoxical elevation of ATP despite the constrained blood supply. It is noteworthy that the NADH elevation in the ischemic region is clearly demarcated by the ATP-depleting core. Results suggest that metabolism in ischemic penumbra does not respond passively to compromised circulation, but actively compensates energy charges.",

author = "Katsuji Hattori and Mayumi Kajimura and Takako Hishiki and Tsuyoshi Nakanishi and Akiko Kubo and Yoshiko Nagahata and Mitsuyo Ohmura and Ayako Yachie-Kinoshita and Tomomi Matsuura and Takayuki Morikawa and Tomomi Nakamura and Mitsutoshi Setou and Makoto Suematsu",

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AU - Hattori, Katsuji

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AU - Kubo, Akiko

AU - Nagahata, Yoshiko

AU - Ohmura, Mitsuyo

AU - Yachie-Kinoshita, Ayako

AU - Matsuura, Tomomi

AU - Morikawa, Takayuki

AU - Nakamura, Tomomi

AU - Setou, Mitsutoshi

AU - Suematsu, Makoto

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AB - Local responses of energy metabolism during brain ischemia are too heterogeneous to decipher redox distribution between anoxic core and adjacent salvageable regions such as penumbra. Imaging mass spectrometry combined by capillary electrophoresis/mass spectrometry providing quantitative metabolomics revealed spatio-temporal changes in adenylates and NADH in a mouse middle-cerebral artery occlusion model. Unlike the core where ATP decreased, the penumbra displayed paradoxical elevation of ATP despite the constrained blood supply. It is noteworthy that the NADH elevation in the ischemic region is clearly demarcated by the ATP-depleting core. Results suggest that metabolism in ischemic penumbra does not respond passively to compromised circulation, but actively compensates energy charges.

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Paradoxical ATP elevation in ischemic penumbra revealed by quantitative imaging mass spectrometry

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