TY - GEN
T1 - Parallel recognition of single-stranded DNA using a biological nanopore array
AU - Tsuji, Y.
AU - Kawano, R.
AU - Osaki, T.
AU - Sasaki, H.
AU - Miki, N.
AU - Takeuchi, S.
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2011
Y1 - 2011
N2 - This paper describes simultaneous detections of a single DNA molecule using a biological nanopore in an array device (Figure 1a). In the previous report, we have successfully reconstituted α-hemolysin αHL) in bilayer lipid membranes (BLMs) using droplets contacting method[1]. In this research, we attempted to detect single-stranded DNA (ssDNA) translocation events through the protein channel. As a result, we succeeded in observing ssDNA translocation events in 15ch simultaneously. We believe that this method can be applied to an automated and high-throughput DNA analysis.
AB - This paper describes simultaneous detections of a single DNA molecule using a biological nanopore in an array device (Figure 1a). In the previous report, we have successfully reconstituted α-hemolysin αHL) in bilayer lipid membranes (BLMs) using droplets contacting method[1]. In this research, we attempted to detect single-stranded DNA (ssDNA) translocation events through the protein channel. As a result, we succeeded in observing ssDNA translocation events in 15ch simultaneously. We believe that this method can be applied to an automated and high-throughput DNA analysis.
KW - Bilayer lipid membranes (BLMs)
KW - Droplets contacting method
KW - Single-stranded DNA (ssDNA)
KW - α-Hemolysin αHL)
UR - http://www.scopus.com/inward/record.url?scp=84883785677&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883785677&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883785677
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 1284
EP - 1286
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -