Parathyroid hormone 1 (1-34) acts on the scales and involves calcium metabolism in goldfish

Nobuo Suzuki; Janine A. Danks; Yusuke Maruyama; Mika Ikegame; Yuichi Sasayama; Atsuhiko Hattori; Masahisa Nakamura; Makoto J. Tabata; Toshio Yamamoto; Ryo Furuya; Kiyofumi Saijoh; Hiroyuki Mishima; Ajai K. Srivastav; Yukihiro Furusawa; Takashi Kondo; Yoshiaki Tabuchi; Ichiro Takasaki; Vishwajit S. Chowdhury; Kazuichi Hayakawa; T. John Martin

doi:10.1016/j.bone.2011.02.004

Parathyroid hormone 1 (1-34) acts on the scales and involves calcium metabolism in goldfish

Nobuo Suzuki, Janine A. Danks, Yusuke Maruyama, Mika Ikegame, Yuichi Sasayama, Atsuhiko Hattori, Masahisa Nakamura, Makoto J. Tabata, Toshio Yamamoto, Ryo Furuya, Kiyofumi Saijoh, Hiroyuki Mishima, Ajai K. Srivastav, Yukihiro Furusawa, Takashi Kondo, Yoshiaki Tabuchi, Ichiro Takasaki, Vishwajit S. Chowdhury, Kazuichi Hayakawa, T. John Martin

School of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

64 Citations (Scopus)

Abstract

The effect of fugu parathyroid hormone 1 (fugu PTH1) on osteoblasts and osteoclasts in teleosts was examined with an assay system using teleost scale and the following markers: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Synthetic fugu PTH1 (1-34) (100. pg/ml-10. ng/ml) significantly increased ALP activity at 6. h of incubation. High-dose (10. ng/ml) fugu PTH1 significantly increased ALP activity even after 18. h of incubation. In the case of TRAP activity, fugu PTH1 did not change at 6. h of incubation, but fugu PTH1 (100. pg/ml-10. ng/ml) significantly increased TRAP activity at 18. h. Similar results were obtained for human PTH (1-34), but there was an even greater response with fugu PTH1 than with human PTH. In vitro, we demonstrated that both the receptor activator of the NF-κB ligand in osteoblasts and the receptor activator NF-κB mRNA expression in osteoclasts increased significantly by fugu PTH1 treatment. In an in vivo experiment, fugu PTH1 induced hypercalcemia resulted from the increase of both osteoblastic and osteoclastic activities in the scale as well as the decrease of scale calcium contents after fugu PTH1 injection. In addition, an in vitro experiment with intramuscular autotransplanted scale indicated that the ratio of multinucleated osteoclasts/mononucleated osteoclasts in PTH-treated scales was significantly higher than that in the control scales. Thus, we concluded that PTH acts on osteoblasts and osteoclasts in the scales and regulates calcium metabolism in goldfish.

Original language	English
Pages (from-to)	1186-1193
Number of pages	8
Journal	Bone
Volume	48
Issue number	5
DOIs	https://doi.org/10.1016/j.bone.2011.02.004
Publication status	Published - 2011 May 1

Keywords

Calcium metabolism
Fish scales
Osteoblasts
Osteoclasts
Parathyroid hormone

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Physiology
Histology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bone.2011.02.004

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Suzuki, N., Danks, J. A., Maruyama, Y., Ikegame, M., Sasayama, Y., Hattori, A., Nakamura, M., Tabata, M. J., Yamamoto, T., Furuya, R., Saijoh, K., Mishima, H., Srivastav, A. K., Furusawa, Y., Kondo, T., Tabuchi, Y., Takasaki, I., Chowdhury, V. S., Hayakawa, K., & Martin, T. J. (2011). Parathyroid hormone 1 (1-34) acts on the scales and involves calcium metabolism in goldfish. Bone, 48(5), 1186-1193. https://doi.org/10.1016/j.bone.2011.02.004

Suzuki, N, Danks, JA, Maruyama, Y, Ikegame, M, Sasayama, Y, Hattori, A, Nakamura, M, Tabata, MJ, Yamamoto, T, Furuya, R, Saijoh, K, Mishima, H, Srivastav, AK, Furusawa, Y, Kondo, T, Tabuchi, Y, Takasaki, I, Chowdhury, VS, Hayakawa, K & Martin, TJ 2011, 'Parathyroid hormone 1 (1-34) acts on the scales and involves calcium metabolism in goldfish', Bone, vol. 48, no. 5, pp. 1186-1193. https://doi.org/10.1016/j.bone.2011.02.004

@article{7ce634a2ed54431ea65bed16821e3701,

title = "Parathyroid hormone 1 (1-34) acts on the scales and involves calcium metabolism in goldfish",

abstract = "The effect of fugu parathyroid hormone 1 (fugu PTH1) on osteoblasts and osteoclasts in teleosts was examined with an assay system using teleost scale and the following markers: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Synthetic fugu PTH1 (1-34) (100. pg/ml-10. ng/ml) significantly increased ALP activity at 6. h of incubation. High-dose (10. ng/ml) fugu PTH1 significantly increased ALP activity even after 18. h of incubation. In the case of TRAP activity, fugu PTH1 did not change at 6. h of incubation, but fugu PTH1 (100. pg/ml-10. ng/ml) significantly increased TRAP activity at 18. h. Similar results were obtained for human PTH (1-34), but there was an even greater response with fugu PTH1 than with human PTH. In vitro, we demonstrated that both the receptor activator of the NF-κB ligand in osteoblasts and the receptor activator NF-κB mRNA expression in osteoclasts increased significantly by fugu PTH1 treatment. In an in vivo experiment, fugu PTH1 induced hypercalcemia resulted from the increase of both osteoblastic and osteoclastic activities in the scale as well as the decrease of scale calcium contents after fugu PTH1 injection. In addition, an in vitro experiment with intramuscular autotransplanted scale indicated that the ratio of multinucleated osteoclasts/mononucleated osteoclasts in PTH-treated scales was significantly higher than that in the control scales. Thus, we concluded that PTH acts on osteoblasts and osteoclasts in the scales and regulates calcium metabolism in goldfish.",

keywords = "Calcium metabolism, Fish scales, Osteoblasts, Osteoclasts, Parathyroid hormone",

author = "Nobuo Suzuki and Danks, {Janine A.} and Yusuke Maruyama and Mika Ikegame and Yuichi Sasayama and Atsuhiko Hattori and Masahisa Nakamura and Tabata, {Makoto J.} and Toshio Yamamoto and Ryo Furuya and Kiyofumi Saijoh and Hiroyuki Mishima and Srivastav, {Ajai K.} and Yukihiro Furusawa and Takashi Kondo and Yoshiaki Tabuchi and Ichiro Takasaki and Chowdhury, {Vishwajit S.} and Kazuichi Hayakawa and Martin, {T. John}",

note = "Funding Information: This study was supported in part by grants to N.S. [Kurita Water and Environment Foundation; Grant-in-Aid for Scientific Research (C) No. 21500404 by JSPS ; Grant-in-Aid for Space Utilization by Japan Aerospace Exploration Agency ], to A.H. [Grant-in-Aid for Scientific Research (C) No. 21570062 by JSPS ], to H.M. [Grant-in-Aid for Scientific Research (C) No. 20592168 by JSPS ], and to K.H. [Grant-in-Aid for Scientific Research of ExTEND2005 and the Environment Research and Technology Development Fund (B-0905) by the Ministry of the Environment in Japan ; Health and Labour Sciences Research Grants of Ministry of Health, Labour and Welfare, Japan ; Grant-in-Aid for Scientific Research (B) No. 21390034 and for Exploratory Research No. 21651018 by JSPS ].",

year = "2011",

month = may,

day = "1",

doi = "10.1016/j.bone.2011.02.004",

language = "English",

volume = "48",

pages = "1186--1193",

journal = "Bone",

issn = "8756-3282",

publisher = "Elsevier Inc.",

number = "5",

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TY - JOUR

T1 - Parathyroid hormone 1 (1-34) acts on the scales and involves calcium metabolism in goldfish

AU - Suzuki, Nobuo

AU - Danks, Janine A.

AU - Maruyama, Yusuke

AU - Ikegame, Mika

AU - Sasayama, Yuichi

AU - Hattori, Atsuhiko

AU - Nakamura, Masahisa

AU - Tabata, Makoto J.

AU - Yamamoto, Toshio

AU - Furuya, Ryo

AU - Saijoh, Kiyofumi

AU - Mishima, Hiroyuki

AU - Srivastav, Ajai K.

AU - Furusawa, Yukihiro

AU - Kondo, Takashi

AU - Tabuchi, Yoshiaki

AU - Takasaki, Ichiro

AU - Chowdhury, Vishwajit S.

AU - Hayakawa, Kazuichi

AU - Martin, T. John

N1 - Funding Information: This study was supported in part by grants to N.S. [Kurita Water and Environment Foundation; Grant-in-Aid for Scientific Research (C) No. 21500404 by JSPS ; Grant-in-Aid for Space Utilization by Japan Aerospace Exploration Agency ], to A.H. [Grant-in-Aid for Scientific Research (C) No. 21570062 by JSPS ], to H.M. [Grant-in-Aid for Scientific Research (C) No. 20592168 by JSPS ], and to K.H. [Grant-in-Aid for Scientific Research of ExTEND2005 and the Environment Research and Technology Development Fund (B-0905) by the Ministry of the Environment in Japan ; Health and Labour Sciences Research Grants of Ministry of Health, Labour and Welfare, Japan ; Grant-in-Aid for Scientific Research (B) No. 21390034 and for Exploratory Research No. 21651018 by JSPS ].

PY - 2011/5/1

Y1 - 2011/5/1

N2 - The effect of fugu parathyroid hormone 1 (fugu PTH1) on osteoblasts and osteoclasts in teleosts was examined with an assay system using teleost scale and the following markers: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Synthetic fugu PTH1 (1-34) (100. pg/ml-10. ng/ml) significantly increased ALP activity at 6. h of incubation. High-dose (10. ng/ml) fugu PTH1 significantly increased ALP activity even after 18. h of incubation. In the case of TRAP activity, fugu PTH1 did not change at 6. h of incubation, but fugu PTH1 (100. pg/ml-10. ng/ml) significantly increased TRAP activity at 18. h. Similar results were obtained for human PTH (1-34), but there was an even greater response with fugu PTH1 than with human PTH. In vitro, we demonstrated that both the receptor activator of the NF-κB ligand in osteoblasts and the receptor activator NF-κB mRNA expression in osteoclasts increased significantly by fugu PTH1 treatment. In an in vivo experiment, fugu PTH1 induced hypercalcemia resulted from the increase of both osteoblastic and osteoclastic activities in the scale as well as the decrease of scale calcium contents after fugu PTH1 injection. In addition, an in vitro experiment with intramuscular autotransplanted scale indicated that the ratio of multinucleated osteoclasts/mononucleated osteoclasts in PTH-treated scales was significantly higher than that in the control scales. Thus, we concluded that PTH acts on osteoblasts and osteoclasts in the scales and regulates calcium metabolism in goldfish.

AB - The effect of fugu parathyroid hormone 1 (fugu PTH1) on osteoblasts and osteoclasts in teleosts was examined with an assay system using teleost scale and the following markers: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Synthetic fugu PTH1 (1-34) (100. pg/ml-10. ng/ml) significantly increased ALP activity at 6. h of incubation. High-dose (10. ng/ml) fugu PTH1 significantly increased ALP activity even after 18. h of incubation. In the case of TRAP activity, fugu PTH1 did not change at 6. h of incubation, but fugu PTH1 (100. pg/ml-10. ng/ml) significantly increased TRAP activity at 18. h. Similar results were obtained for human PTH (1-34), but there was an even greater response with fugu PTH1 than with human PTH. In vitro, we demonstrated that both the receptor activator of the NF-κB ligand in osteoblasts and the receptor activator NF-κB mRNA expression in osteoclasts increased significantly by fugu PTH1 treatment. In an in vivo experiment, fugu PTH1 induced hypercalcemia resulted from the increase of both osteoblastic and osteoclastic activities in the scale as well as the decrease of scale calcium contents after fugu PTH1 injection. In addition, an in vitro experiment with intramuscular autotransplanted scale indicated that the ratio of multinucleated osteoclasts/mononucleated osteoclasts in PTH-treated scales was significantly higher than that in the control scales. Thus, we concluded that PTH acts on osteoblasts and osteoclasts in the scales and regulates calcium metabolism in goldfish.

KW - Calcium metabolism

KW - Fish scales

KW - Osteoblasts

KW - Osteoclasts

KW - Parathyroid hormone

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VL - 48

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JO - Bone

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ER -

Parathyroid hormone 1 (1-34) acts on the scales and involves calcium metabolism in goldfish

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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