TY - JOUR
T1 - PARP-1 regulates expression of TGF-β receptors in T cells
AU - Zhang, Pin
AU - Nakatsukasa, Hiroko
AU - Tu, Eric
AU - Kasagi, Shimpei
AU - Cui, Kairong
AU - Ishikawa, Masaki
AU - Konkel, Joanne E.
AU - Maruyama, Takashi
AU - Wei, Gang
AU - Abbatiello, Brittany
AU - Wang, Zhao Qi
AU - Zhao, Keji
AU - Chen, Wan Jun
N1 - Funding Information:
This work was supported by the Intramural Research Program of the National Institute of Dental and Craniofacial Research (National Institutes of Health).
Publisher Copyright:
© 2013, American Society of Hematology. All rights reserved.
PY - 2013
Y1 - 2013
N2 - Transforming growth factor-b (TGF-b) receptors (TbRs) are essential components for TGF-b signal transduction in T cells, yet the mechanisms by which the receptors are regulated remain poorly understood. We show here that Poly(ADP-ribose) polymerase-1 (PARP-1) regulates TGF-b receptor I (TbRI) and II (TbRII) expression in CD4+ T cells and subsequently affects Smad2/3-mediated TGF-b signal transduction. Inhibition of PARP-1 led to the upregulation of both TbRI and TbRII, yet the underlying molecular mechanisms were distinct. PARP-1 selectively bound to the promoter of TbRII, whereas the enzymatic activity of PARP-1 was responsible for the inhibition of TbRI expression. Importantly, inhibition of PARP-1 also enhanced expression of TbRs in human CD4+ T cells. Thus, PARP-1 regulates TbR expression and TGF-b signaling in T cells.
AB - Transforming growth factor-b (TGF-b) receptors (TbRs) are essential components for TGF-b signal transduction in T cells, yet the mechanisms by which the receptors are regulated remain poorly understood. We show here that Poly(ADP-ribose) polymerase-1 (PARP-1) regulates TGF-b receptor I (TbRI) and II (TbRII) expression in CD4+ T cells and subsequently affects Smad2/3-mediated TGF-b signal transduction. Inhibition of PARP-1 led to the upregulation of both TbRI and TbRII, yet the underlying molecular mechanisms were distinct. PARP-1 selectively bound to the promoter of TbRII, whereas the enzymatic activity of PARP-1 was responsible for the inhibition of TbRI expression. Importantly, inhibition of PARP-1 also enhanced expression of TbRs in human CD4+ T cells. Thus, PARP-1 regulates TbR expression and TGF-b signaling in T cells.
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U2 - 10.1182/blood-2013-05-503250
DO - 10.1182/blood-2013-05-503250
M3 - Article
C2 - 23940283
AN - SCOPUS:84887365846
SN - 0006-4971
VL - 122
SP - 2224
EP - 2232
JO - Blood
JF - Blood
IS - 13
ER -