Abstract
A thymidine phosphorylase (TP) preparation was partially purified from human gastric cancer (poorly differentiated adenocarcinoma). The specific activity of the final preparation represented a 379-fold purification of the 7000g supernatant of tissue homogenate. The phosphorolytic activities toward thymidine (dThd), 5’-deoxy-5-fluorouridine (5’-DFUR), and l-(tetrahydro-2-furanyl)-5-fluorouracil (Tegafur) remained closely in parallel during the whole purification procedure. The results provide evidence in support of the assumption that 5’-DFUR and Tegafur are converted into 5-fiuorouracil, an activated form of the antitumor agents, in human tumor tissues by a TP activity. The values of Kmof the TP preparation were 1.68 x 10~4, 1.72 x 10–3, 1.33 x 10–2, and 4.76 x 10-2 m for dThd, 5’-DFUR, Tegafur, and uridine, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 1219-1222 |
| Number of pages | 4 |
| Journal | Chemical and Pharmaceutical Bulletin |
| Volume | 34 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1986 Jan 1 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- 5-fluorouracil
- 5’-deoxy-5-fluorouridine
- gastric cancer
- human tumor
- l-(tetrahydro-2-furanyl)-5-fluorouracil
- thymidine
- thymidine phosphorylase
- uridine
- uridine phosphorylase
ASJC Scopus subject areas
- General Chemistry
- Drug Discovery
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