Partition coefficients of polyphenols for phosphatidylcholine investigated by HPLC with an immobilized artificial membrane column

Yoshinori Uekusa; Yuko Takeshita; Takeshi Ishii; Tsutomu Nakayama

doi:10.1271/bbb.80435

Partition coefficients of polyphenols for phosphatidylcholine investigated by HPLC with an immobilized artificial membrane column

Yoshinori Uekusa, Yuko Takeshita, Takeshi Ishii, Tsutomu Nakayama

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The biological activity of polyphenols in vitro reflects their affinity for cell membranes and the amount of cellular incorporation. The interaction of polyphenols with phosphatidylcholine was investigated by HPLC with an immobilized artificial membrane (IAM) column. The IAM partition coefficients (K_IAM) of the polyphenols, calculated by retention times, correlated well with the amounts of polyphenols incorporation into the liposomes.

Original language	English
Pages (from-to)	3289-3292
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	72
Issue number	12
DOIs	https://doi.org/10.1271/bbb.80435
Publication status	Published - 2008
Externally published	Yes

Keywords

HPLC
IAM partition coefficient (K)
Immobilized artificial membrane (IAM)
Lipid membranes
Polyphenol

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.80435

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title = "Partition coefficients of polyphenols for phosphatidylcholine investigated by HPLC with an immobilized artificial membrane column",

abstract = "The biological activity of polyphenols in vitro reflects their affinity for cell membranes and the amount of cellular incorporation. The interaction of polyphenols with phosphatidylcholine was investigated by HPLC with an immobilized artificial membrane (IAM) column. The IAM partition coefficients (KIAM) of the polyphenols, calculated by retention times, correlated well with the amounts of polyphenols incorporation into the liposomes.",

keywords = "HPLC, IAM partition coefficient (K), Immobilized artificial membrane (IAM), Lipid membranes, Polyphenol",

author = "Yoshinori Uekusa and Yuko Takeshita and Takeshi Ishii and Tsutomu Nakayama",

note = "Funding Information: This work was supported by a Grant-in-Aid, no. 19580147, for Scientific Research and by the Global COE Program of the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and by a Sasakawa Scientific Research Grant from the Japan Science Society.",

year = "2008",

doi = "10.1271/bbb.80435",

language = "English",

volume = "72",

pages = "3289--3292",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "12",

}

TY - JOUR

T1 - Partition coefficients of polyphenols for phosphatidylcholine investigated by HPLC with an immobilized artificial membrane column

AU - Uekusa, Yoshinori

AU - Takeshita, Yuko

AU - Ishii, Takeshi

AU - Nakayama, Tsutomu

N1 - Funding Information: This work was supported by a Grant-in-Aid, no. 19580147, for Scientific Research and by the Global COE Program of the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and by a Sasakawa Scientific Research Grant from the Japan Science Society.

PY - 2008

Y1 - 2008

N2 - The biological activity of polyphenols in vitro reflects their affinity for cell membranes and the amount of cellular incorporation. The interaction of polyphenols with phosphatidylcholine was investigated by HPLC with an immobilized artificial membrane (IAM) column. The IAM partition coefficients (KIAM) of the polyphenols, calculated by retention times, correlated well with the amounts of polyphenols incorporation into the liposomes.

AB - The biological activity of polyphenols in vitro reflects their affinity for cell membranes and the amount of cellular incorporation. The interaction of polyphenols with phosphatidylcholine was investigated by HPLC with an immobilized artificial membrane (IAM) column. The IAM partition coefficients (KIAM) of the polyphenols, calculated by retention times, correlated well with the amounts of polyphenols incorporation into the liposomes.

KW - HPLC

KW - IAM partition coefficient (K)

KW - Immobilized artificial membrane (IAM)

KW - Lipid membranes

KW - Polyphenol

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U2 - 10.1271/bbb.80435

DO - 10.1271/bbb.80435

M3 - Article

C2 - 19060387

AN - SCOPUS:58149094691

SN - 0916-8451

VL - 72

SP - 3289

EP - 3292

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 12

ER -

Partition coefficients of polyphenols for phosphatidylcholine investigated by HPLC with an immobilized artificial membrane column

Abstract

Keywords

ASJC Scopus subject areas

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