TY - JOUR
T1 - Parylene-coating in PDMS microfluidic channels prevents the absorption of fluorescent dyes
AU - Sasaki, Hirotaka
AU - Onoe, Hiroaki
AU - Osaki, Toshihisa
AU - Kawano, Ryuji
AU - Takeuchi, Shoji
N1 - Funding Information:
Shoji Takeuchi received his Dr. Eng. degree in mechanical engineering in 2000 from the University of Tokyo. He is currently an Associate Professor in the Center for International Research on Micromechatronics (CIRMM), Institute of Industrial Science (IIS), The University of Tokyo. He has received several awards including the Advanced Research from the Japan Society of Medical Electronics and Biological Engineering in 2001, Young Scientists’ Prize, the Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology in 2008, and the JSPS prize from the Japan Society for the Promotion of Science in 2010.
PY - 2010/10/21
Y1 - 2010/10/21
N2 - Absorption of fluorescent molecules is a troublesome property of microfluidic devices fabricated with PDMS. The absorption raises background-levels of fluorescence from PDMS, and reduces concentrations of injected fluorescent molecules in PDMS channels. We report here that conformal deposition of poly-p-xylylene derivatives (parylenes) on the surface of PDMS substantially suppressed the absorption of Rhodamine B. In a test of this technique, we found parylene-coated PDMS channels to be useful in accurate analyses using fluorescence intensity.
AB - Absorption of fluorescent molecules is a troublesome property of microfluidic devices fabricated with PDMS. The absorption raises background-levels of fluorescence from PDMS, and reduces concentrations of injected fluorescent molecules in PDMS channels. We report here that conformal deposition of poly-p-xylylene derivatives (parylenes) on the surface of PDMS substantially suppressed the absorption of Rhodamine B. In a test of this technique, we found parylene-coated PDMS channels to be useful in accurate analyses using fluorescence intensity.
KW - Absorption
KW - Fluorescence
KW - Microfluidic channel
KW - Parylene
KW - Poly(dimethylsiloxane)
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U2 - 10.1016/j.snb.2010.07.021
DO - 10.1016/j.snb.2010.07.021
M3 - Article
AN - SCOPUS:79960936548
SN - 0925-4005
VL - 150
SP - 478
EP - 482
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
IS - 1
ER -