Parylene-coating in PDMS microfluidic channels prevents the absorption of fluorescent dyes

Hirotaka Sasaki, Hiroaki Onoe, Toshihisa Osaki, Ryuji Kawano, Shoji Takeuchi

Research output: Contribution to journalArticlepeer-review

94 Citations (Scopus)


Absorption of fluorescent molecules is a troublesome property of microfluidic devices fabricated with PDMS. The absorption raises background-levels of fluorescence from PDMS, and reduces concentrations of injected fluorescent molecules in PDMS channels. We report here that conformal deposition of poly-p-xylylene derivatives (parylenes) on the surface of PDMS substantially suppressed the absorption of Rhodamine B. In a test of this technique, we found parylene-coated PDMS channels to be useful in accurate analyses using fluorescence intensity.

Original languageEnglish
Pages (from-to)478-482
Number of pages5
JournalSensors and Actuators, B: Chemical
Issue number1
Publication statusPublished - 2010 Oct 21
Externally publishedYes


  • Absorption
  • Fluorescence
  • Microfluidic channel
  • Parylene
  • Poly(dimethylsiloxane)

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry


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