Pathogens in COPD exacerbations identified by comprehensive real-time PCR plus older methods

Kenichiro Shimizu; Yutaka Yoshii; Miyuki Morozumi; Naoko Chiba; Kimiko Ubukata; Hironori Uruga; Shigeo Hanada; Nayuta Saito; Tsukasa Kadota; Saburo Ito; Hiroshi Wakui; Naoki Takasaka; Shunsuke Minagawa; Jun Kojima; Hiromichi Hara; Takanori Numata; Makoto Kawaishi; Keisuke Saito; Jun Araya; Yumi Kaneko; Katsutoshi Nakayama; Kazuma Kishi; Kazuyoshi Kuwano

doi:10.2147/COPD.S82752

Pathogens in COPD exacerbations identified by comprehensive real-time PCR plus older methods

Kenichiro Shimizu, Yutaka Yoshii, Miyuki Morozumi, Naoko Chiba, Kimiko Ubukata, Hironori Uruga, Shigeo Hanada, Nayuta Saito, Tsukasa Kadota, Saburo Ito, Hiroshi Wakui, Naoki Takasaka, Shunsuke Minagawa, Jun Kojima, Hiromichi Hara, Takanori Numata, Makoto Kawaishi, Keisuke Saito, Jun Araya, Yumi KanekoKatsutoshi Nakayama, Kazuma Kishi, Kazuyoshi Kuwano

Department of Infectious Diseases

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Respiratory infection is a major cause of exacerbation in chronic obstructive pulmonary disease (COPD). Infectious contributions to exacerbations remain incompletely described. We therefore analyzed respiratory tract samples by comprehensive real-time polymerase chain reaction (PCR) in combination with conventional methods. We evaluated multiple risk factors for prolonged hospitalization to manage COPD exacerbations, including infectious agents. Over 19 months, we prospectively studied 46 patients with 50 COPD exacerbations, collecting nasopharyngeal swab and sputum samples from each. We carried out real-time PCR designed to detect six bacterial species and eleven viruses, together with conventional procedures, including sputum culture. Infectious etiologies of COPD exacerbations were identified in 44 of 50 exacerbations (88%). Infections were viral in 17 of 50 exacerbations (34%). COPD exacerbations caused by Gram-negative bacilli, including enteric and nonfermenting organisms, were significantly associated with prolonged hospitalization for COPD exacerbations. Our results support the use of a combination of real-time PCR and conventional methods for determining both infectious etiologies and risk of extended hospitalization.

Original language	English
Pages (from-to)	2009-2016
Number of pages	8
Journal	International Journal of COPD
Volume	10
Issue number	1
DOIs	https://doi.org/10.2147/COPD.S82752
Publication status	Published - 2015 Sept 23

Keywords

COPD
Etiology
Exacerbation
Prolonged hospitalization
Real-time PCR
Risk factor

ASJC Scopus subject areas

Pulmonary and Respiratory Medicine
Health Policy
Public Health, Environmental and Occupational Health

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2147/COPD.S82752

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Shimizu, K., Yoshii, Y., Morozumi, M., Chiba, N., Ubukata, K., Uruga, H., Hanada, S., Saito, N., Kadota, T., Ito, S., Wakui, H., Takasaka, N., Minagawa, S., Kojima, J., Hara, H., Numata, T., Kawaishi, M., Saito, K., Araya, J., ... Kuwano, K. (2015). Pathogens in COPD exacerbations identified by comprehensive real-time PCR plus older methods. International Journal of COPD, 10(1), 2009-2016. https://doi.org/10.2147/COPD.S82752

Shimizu, K, Yoshii, Y, Morozumi, M, Chiba, N, Ubukata, K, Uruga, H, Hanada, S, Saito, N, Kadota, T, Ito, S, Wakui, H, Takasaka, N, Minagawa, S, Kojima, J, Hara, H, Numata, T, Kawaishi, M, Saito, K, Araya, J, Kaneko, Y, Nakayama, K, Kishi, K & Kuwano, K 2015, 'Pathogens in COPD exacerbations identified by comprehensive real-time PCR plus older methods', International Journal of COPD, vol. 10, no. 1, pp. 2009-2016. https://doi.org/10.2147/COPD.S82752

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abstract = "Respiratory infection is a major cause of exacerbation in chronic obstructive pulmonary disease (COPD). Infectious contributions to exacerbations remain incompletely described. We therefore analyzed respiratory tract samples by comprehensive real-time polymerase chain reaction (PCR) in combination with conventional methods. We evaluated multiple risk factors for prolonged hospitalization to manage COPD exacerbations, including infectious agents. Over 19 months, we prospectively studied 46 patients with 50 COPD exacerbations, collecting nasopharyngeal swab and sputum samples from each. We carried out real-time PCR designed to detect six bacterial species and eleven viruses, together with conventional procedures, including sputum culture. Infectious etiologies of COPD exacerbations were identified in 44 of 50 exacerbations (88%). Infections were viral in 17 of 50 exacerbations (34%). COPD exacerbations caused by Gram-negative bacilli, including enteric and nonfermenting organisms, were significantly associated with prolonged hospitalization for COPD exacerbations. Our results support the use of a combination of real-time PCR and conventional methods for determining both infectious etiologies and risk of extended hospitalization.",

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AU - Ubukata, Kimiko

AU - Uruga, Hironori

AU - Hanada, Shigeo

AU - Saito, Nayuta

AU - Kadota, Tsukasa

AU - Ito, Saburo

AU - Wakui, Hiroshi

AU - Takasaka, Naoki

AU - Minagawa, Shunsuke

AU - Kojima, Jun

AU - Hara, Hiromichi

AU - Numata, Takanori

AU - Kawaishi, Makoto

AU - Saito, Keisuke

AU - Araya, Jun

AU - Kaneko, Yumi

AU - Nakayama, Katsutoshi

AU - Kishi, Kazuma

AU - Kuwano, Kazuyoshi

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Pathogens in COPD exacerbations identified by comprehensive real-time PCR plus older methods

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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