Peptides derived from atrial natriuretic polypeptide precursor in human and monkey brains

Hiroshi Itoh, Kazuwa Nakao, Masashi Mukoyama, Kiminori Hosada, Shozo Shiono, Narito Morii, Takayuki Yamada, Akira Sugawara, Yoshiko Saito, Hiroshi Arai, Gotaro Shirakami, Hiroo Imura

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Using radio-immunoassays for the C-terminai sequence of a-atrial natriuretic polypeptide [atrial natriuretic factor (ANF)-(99-126)], α-ANP (17-28) [ANF-(115-126)], which corresponds to γ-human ANP [115-126; - γ -hAN P-(115-126)] and for the N-terminal sequence of γ -hANP [human ANF-(1-126)], γ -hANP-(1-25) [human ANF-(1-25)], we detected the parallel distribution of α-hANP-like immunoreactivity and - γ -hANP-(1- 25)-like immunoreactivity in the human and monkey brains, with the highest concentrations in the midbrain and the pons. High performance gel permeation chromatography coupled with the two radio-immunoassays and also with another radio-immunoassay using a monoclonal antibody against the N-terminal sequence of a-ANP revealed that γ -ANP is synthesized in the brain and cleaved into N-terminally deleted form(s) of α-ANP and 10-K N-terminal fragment(s) of γ -ANP, which coexist within the neuron. These results suggest that the post-translational processing of γ -ANP in the brain is different from that in the heart.

Original languageEnglish
Pages (from-to)S309-S313
JournalJournal of Hypertension, Supplement
Issue number4
Publication statusPublished - 1988 Dec
Externally publishedYes


  • Atrial natriuretic polypeptide
  • Brain
  • Human
  • Monkey
  • Post-translational processing
  • Prohormone

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine


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