Permuted tRNA genes expressed via a circular RNA intermediate in Cyanidioschyzon merolae

Akiko Soma; Akinori Onodera; Junichi Sugahara; Akio Kanai; Nozomu Yachie; Masaru Tomita; Fujio Kawamura; Yasuhiko Sekine

doi:10.1126/science.1145718

Permuted tRNA genes expressed via a circular RNA intermediate in Cyanidioschyzon merolae

Akiko Soma, Akinori Onodera, Junichi Sugahara, Akio Kanai, Nozomu Yachie, Masaru Tomita, Fujio Kawamura, Yasuhiko Sekine

Faculty of Environment and Information Studies

Research output: Contribution to journal › Article › peer-review

87 Citations (Scopus)

Abstract

A computational analysis of the nuclear genome of a red alga, Cyanidioschyzon merolae, identified 11 transfer RNA (tRNA) genes in which the 3′ half of the tRNA lies upstream of the 5′ half in the genome. We verified that these genes are expressed and produce mature tRNAs that are aminoacylated. Analysis of tRNA-processing intermediates for these genes indicates an unusual processing pathway in which the termini of the tRNA precursor are ligated, resulting in formation of a characteristic circular RNA intermediate that is then processed at the acceptor stem to generate the correct termini.

Original language	English
Pages (from-to)	450-453
Number of pages	4
Journal	Science
Volume	318
Issue number	5849
DOIs	https://doi.org/10.1126/science.1145718
Publication status	Published - 2007 Oct 19

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title = "Permuted tRNA genes expressed via a circular RNA intermediate in Cyanidioschyzon merolae",

abstract = "A computational analysis of the nuclear genome of a red alga, Cyanidioschyzon merolae, identified 11 transfer RNA (tRNA) genes in which the 3′ half of the tRNA lies upstream of the 5′ half in the genome. We verified that these genes are expressed and produce mature tRNAs that are aminoacylated. Analysis of tRNA-processing intermediates for these genes indicates an unusual processing pathway in which the termini of the tRNA precursor are ligated, resulting in formation of a characteristic circular RNA intermediate that is then processed at the acceptor stem to generate the correct termini.",

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T1 - Permuted tRNA genes expressed via a circular RNA intermediate in Cyanidioschyzon merolae

AU - Soma, Akiko

AU - Onodera, Akinori

AU - Sugahara, Junichi

AU - Kanai, Akio

AU - Yachie, Nozomu

AU - Tomita, Masaru

AU - Kawamura, Fujio

AU - Sekine, Yasuhiko

PY - 2007/10/19

Y1 - 2007/10/19

N2 - A computational analysis of the nuclear genome of a red alga, Cyanidioschyzon merolae, identified 11 transfer RNA (tRNA) genes in which the 3′ half of the tRNA lies upstream of the 5′ half in the genome. We verified that these genes are expressed and produce mature tRNAs that are aminoacylated. Analysis of tRNA-processing intermediates for these genes indicates an unusual processing pathway in which the termini of the tRNA precursor are ligated, resulting in formation of a characteristic circular RNA intermediate that is then processed at the acceptor stem to generate the correct termini.

AB - A computational analysis of the nuclear genome of a red alga, Cyanidioschyzon merolae, identified 11 transfer RNA (tRNA) genes in which the 3′ half of the tRNA lies upstream of the 5′ half in the genome. We verified that these genes are expressed and produce mature tRNAs that are aminoacylated. Analysis of tRNA-processing intermediates for these genes indicates an unusual processing pathway in which the termini of the tRNA precursor are ligated, resulting in formation of a characteristic circular RNA intermediate that is then processed at the acceptor stem to generate the correct termini.

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Permuted tRNA genes expressed via a circular RNA intermediate in Cyanidioschyzon merolae

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