Phase II enzyme induction by a carotenoid, lutein, in a PC12D neuronal cell line

Seiji Miyake, Saori Kobayashi, Kazuo Tsubota, Yoko Ozawa

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


The mechanism by which lutein, a carotenoid, acts as an antioxidant in retinal cells is still not fully understood. Here, lutein treatment of a neuronal cell line (PC12D) immediately resulted in reduced intracellular ROS levels, implying that it has a direct role in ROS scavenging. Significantly, lutein treatment also induced phase II antioxidative enzyme expression, probably via a nuclear factor-like 2 (Nrf2) independent pathway. This latter mechanism could explain why lutein acts diversely to protect against oxidative/cytotoxic stress, and why it is physiologically involved in the human neural tissue, such as the retina.

Original languageEnglish
Pages (from-to)535-540
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 2014 Apr 4
Externally publishedYes


  • Antioxidant enzymes
  • Lutein
  • Nrf2
  • PC12D
  • Reactive oxygen species

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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