Photo-cross-linked small-molecule affinity matrix for facilitating forward and reverse chemical genetics

Naoki Kanoh; Kaori Honda; Sim Simizu; Makoto Muroi; Hiroyuki Osada

doi:10.1002/anie.200462370

Photo-cross-linked small-molecule affinity matrix for facilitating forward and reverse chemical genetics

Naoki Kanoh, Kaori Honda, Sim Simizu, Makoto Muroi, Hiroyuki Osada

Research output: Contribution to journal › Article › peer-review

103 Citations (Scopus)

Abstract

(Figure Presented) A photoaffinity reaction has been used to photo-cross-link small molecules on agarose beads to identify and purify the binding proteins (see picture; PMF: peptide mass fingerprinting). This technology is considered potentially useful for the confirmation of specificity between a small molecule and a protein of interest in the field of reverse chemical genetics.

Original language	English
Pages (from-to)	3559-3562
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	44
Issue number	23
DOIs	https://doi.org/10.1002/anie.200462370
Publication status	Published - 2005 Jun 6
Externally published	Yes

Keywords

Affinity purification
Immobilization
Molecular targeting
Peptides
Photochemistry

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/anie.200462370

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abstract = "(Figure Presented) A photoaffinity reaction has been used to photo-cross-link small molecules on agarose beads to identify and purify the binding proteins (see picture; PMF: peptide mass fingerprinting). This technology is considered potentially useful for the confirmation of specificity between a small molecule and a protein of interest in the field of reverse chemical genetics.",

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author = "Naoki Kanoh and Kaori Honda and Sim Simizu and Makoto Muroi and Hiroyuki Osada",

year = "2005",

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AU - Kanoh, Naoki

AU - Honda, Kaori

AU - Simizu, Sim

AU - Muroi, Makoto

AU - Osada, Hiroyuki

PY - 2005/6/6

Y1 - 2005/6/6

N2 - (Figure Presented) A photoaffinity reaction has been used to photo-cross-link small molecules on agarose beads to identify and purify the binding proteins (see picture; PMF: peptide mass fingerprinting). This technology is considered potentially useful for the confirmation of specificity between a small molecule and a protein of interest in the field of reverse chemical genetics.

AB - (Figure Presented) A photoaffinity reaction has been used to photo-cross-link small molecules on agarose beads to identify and purify the binding proteins (see picture; PMF: peptide mass fingerprinting). This technology is considered potentially useful for the confirmation of specificity between a small molecule and a protein of interest in the field of reverse chemical genetics.

KW - Affinity purification

KW - Immobilization

KW - Molecular targeting

KW - Peptides

KW - Photochemistry

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ER -

Photo-cross-linked small-molecule affinity matrix for facilitating forward and reverse chemical genetics

Abstract

Keywords

ASJC Scopus subject areas

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