PH/temperature-responsive fluorescence polymer probe with pH-controlled cellular uptake

Yuki Hiruta, Takaaki Funatsu, Minami Matsuura, Jian Wang, Eri Ayano, Hideko Kanazawa

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)


A pH/temperature-responsive fluorescence polymer, I42D50S8-FL, consisting of N-isopropylacrylamide, N,N-dimethylacrylamide, and sulfamethazineacrylamide was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization, followed by activation of terminal carboxyl groups with N-hydroxysuccinimide and reaction with 5-aminofluorescein (FL). I42D50S8-FL exhibited a pH-dependent phase transition across the lower critical solution temperature (LCST), with specific intracellular uptake observed only in weak acidic conditions. I42D50S8-FL has the potential to be applied for the selective imaging of acidic tumor microenvironments.

Original languageEnglish
Pages (from-to)724-731
Number of pages8
JournalSensors and Actuators, B: Chemical
Issue numberPartA
Publication statusPublished - 2015 Feb


  • Acidic tumor microenvironment
  • Cellular uptake
  • Fluorescence polymer
  • pH

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry


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