PH/temperature-responsive fluorescence polymer probe with pH-controlled cellular uptake

Yuki Hiruta; Takaaki Funatsu; Minami Matsuura; Jian Wang; Eri Ayano; Hideko Kanazawa

doi:10.1016/j.snb.2014.10.065

PH/temperature-responsive fluorescence polymer probe with pH-controlled cellular uptake

Yuki Hiruta, Takaaki Funatsu, Minami Matsuura, Jian Wang, Eri Ayano, Hideko Kanazawa

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

A pH/temperature-responsive fluorescence polymer, I₄₂D₅₀S₈-FL, consisting of N-isopropylacrylamide, N,N-dimethylacrylamide, and sulfamethazineacrylamide was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization, followed by activation of terminal carboxyl groups with N-hydroxysuccinimide and reaction with 5-aminofluorescein (FL). I₄₂D₅₀S₈-FL exhibited a pH-dependent phase transition across the lower critical solution temperature (LCST), with specific intracellular uptake observed only in weak acidic conditions. I₄₂D₅₀S₈-FL has the potential to be applied for the selective imaging of acidic tumor microenvironments.

Original language	English
Pages (from-to)	724-731
Number of pages	8
Journal	Sensors and Actuators, B: Chemical
Volume	207
Issue number	PartA
DOIs	https://doi.org/10.1016/j.snb.2014.10.065
Publication status	Published - 2015 Feb

Keywords

Acidic tumor microenvironment
Cellular uptake
Fluorescence polymer
pH

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2014.10.065

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title = "PH/temperature-responsive fluorescence polymer probe with pH-controlled cellular uptake",

abstract = "A pH/temperature-responsive fluorescence polymer, I42D50S8-FL, consisting of N-isopropylacrylamide, N,N-dimethylacrylamide, and sulfamethazineacrylamide was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization, followed by activation of terminal carboxyl groups with N-hydroxysuccinimide and reaction with 5-aminofluorescein (FL). I42D50S8-FL exhibited a pH-dependent phase transition across the lower critical solution temperature (LCST), with specific intracellular uptake observed only in weak acidic conditions. I42D50S8-FL has the potential to be applied for the selective imaging of acidic tumor microenvironments.",

keywords = "Acidic tumor microenvironment, Cellular uptake, Fluorescence polymer, pH",

author = "Yuki Hiruta and Takaaki Funatsu and Minami Matsuura and Jian Wang and Eri Ayano and Hideko Kanazawa",

note = "Funding Information: This work was supported in part by Grant-in-Aid for Research Activity Start-Up (Grant No. 25893232 ) from Japan Society for Promotion of Science (JSPS). ",

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doi = "10.1016/j.snb.2014.10.065",

language = "English",

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journal = "Sensors and Actuators, B: Chemical",

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T1 - PH/temperature-responsive fluorescence polymer probe with pH-controlled cellular uptake

AU - Hiruta, Yuki

AU - Funatsu, Takaaki

AU - Matsuura, Minami

AU - Wang, Jian

AU - Ayano, Eri

AU - Kanazawa, Hideko

N1 - Funding Information: This work was supported in part by Grant-in-Aid for Research Activity Start-Up (Grant No. 25893232 ) from Japan Society for Promotion of Science (JSPS).

PY - 2015/2

Y1 - 2015/2

N2 - A pH/temperature-responsive fluorescence polymer, I42D50S8-FL, consisting of N-isopropylacrylamide, N,N-dimethylacrylamide, and sulfamethazineacrylamide was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization, followed by activation of terminal carboxyl groups with N-hydroxysuccinimide and reaction with 5-aminofluorescein (FL). I42D50S8-FL exhibited a pH-dependent phase transition across the lower critical solution temperature (LCST), with specific intracellular uptake observed only in weak acidic conditions. I42D50S8-FL has the potential to be applied for the selective imaging of acidic tumor microenvironments.

AB - A pH/temperature-responsive fluorescence polymer, I42D50S8-FL, consisting of N-isopropylacrylamide, N,N-dimethylacrylamide, and sulfamethazineacrylamide was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization, followed by activation of terminal carboxyl groups with N-hydroxysuccinimide and reaction with 5-aminofluorescein (FL). I42D50S8-FL exhibited a pH-dependent phase transition across the lower critical solution temperature (LCST), with specific intracellular uptake observed only in weak acidic conditions. I42D50S8-FL has the potential to be applied for the selective imaging of acidic tumor microenvironments.

KW - Acidic tumor microenvironment

KW - Cellular uptake

KW - Fluorescence polymer

KW - pH

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JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

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PH/temperature-responsive fluorescence polymer probe with pH-controlled cellular uptake

Abstract

Keywords

ASJC Scopus subject areas

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