TY - JOUR
T1 - Physicochemical properties of pDNA/chitosan complexes as gene delivery systems
AU - Hagiwara, Kenji
AU - Anastasia, Riany
AU - Nakata, Mitsuhiro
AU - Sato, Toshinori
PY - 2011/12/1
Y1 - 2011/12/1
N2 - Successful gene therapy depends on the development of effective gene carriers. Naturally occurring chitosan has been employed widely as a non-viral gene carrier because of its low toxicity, low immunogenicity, biocompatibility, and biodegradability. In this review, we summarize the utilization of chitosan, modified chitosan, and chitosan-containing ternary complexes as gene carriers. In particular, we discuss the influence of the physicochemical features of pDNA/chitosan complexes on their functions, such as stability and gene transfer into cells.
AB - Successful gene therapy depends on the development of effective gene carriers. Naturally occurring chitosan has been employed widely as a non-viral gene carrier because of its low toxicity, low immunogenicity, biocompatibility, and biodegradability. In this review, we summarize the utilization of chitosan, modified chitosan, and chitosan-containing ternary complexes as gene carriers. In particular, we discuss the influence of the physicochemical features of pDNA/chitosan complexes on their functions, such as stability and gene transfer into cells.
KW - Atomic force microscopy
KW - Cell transfection
KW - Chitosan
KW - DNA complex
KW - Gene delivery system
KW - Intracellular trafficking
KW - Morphology
KW - Sugar-modified chitosan
UR - http://www.scopus.com/inward/record.url?scp=82155191585&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=82155191585&partnerID=8YFLogxK
U2 - 10.2174/157016311798109362
DO - 10.2174/157016311798109362
M3 - Article
C2 - 21726189
AN - SCOPUS:82155191585
SN - 1570-1638
VL - 8
SP - 329
EP - 339
JO - Current Drug Discovery Technologies
JF - Current Drug Discovery Technologies
IS - 4
ER -