Physiological and environmental parameters associated with mass spectrometry-based salivary metabolomic profiles

Masahiro Sugimoto, Juri Saruta, Chisa Matsuki, Masahiro To, Hiromi Onuma, Miku Kaneko, Tomoyoshi Soga, Masaru Tomita, Keiichi Tsukinoki

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)


Mass spectrometry (MS)-based metabolomic methods enable simultaneous profiling of hundreds of salivary metabolites, and may be useful to diagnose a wide range of diseases using saliva. However, few studies have evaluated the effects of physiological or environmental factors on salivary metabolomic profiles. Therefore, we used capillary electrophoresis-MS to analyze saliva metabolite profiles in 155 subjects with reasonable oral hygiene, and examined the effects of physiological and environmental factors on the metabolite profiles. Overall, 257 metabolites were identified and quantified. The global profiles and individual metabolites were evaluated by principle component analysis and univariate tests, respectively. Collection method, collection time, sex, body mass index, and smoking affected the global metabolite profiles. However, age also might contribute to the bias in sex and collection time. The profiles were relatively unaffected by other parameters, such as alcohol consumption and smoking, tooth brushing, or the use of medications or nutritional supplements. Temporomandibular joint disorders had relatively greater effects on salivary metabolites than other dental abnormalities (e. g., stomatitis, tooth alignment, and dental caries). These findings provide further insight into the diversity and stability of salivary metabolomic profiles, as well as the generalizability of disease-specific biomarkers.

Original languageEnglish
Pages (from-to)454-463
Number of pages10
Issue number2
Publication statusPublished - 2013 Apr


  • Capillary electrophoresis
  • Mass spectrometry
  • Metabolomics
  • Oral hygiene
  • Saliva
  • Temporomandibular joint disorders

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Clinical Biochemistry


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