Pilot study of changes in salivary metabolic profiles induced by template therapy

Shoji Tanaka, Hitoshi Taga, Kiyoshi Maehara, Azusa Kaneshima, Mamoru Machino, Hiromi Onuma, Miku Kaneko, Hiroshi Sakagami, Masahiro Sugimoto, Tomoyoshi Soga, Masaru Tomita

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Background: Occlusal raising method (so-called 'Template therapy') has been reported to alleviate various diseases and symptoms, but the underlying mechanism is not clear. We searched the low-molecular weight metabolite(s) in the saliva, the concentration of which is significantly changed by the template therapy. Materials and Methods: One female patient with headache underwent the template therapy for 12 days, and her total saliva was subjected to non-targeted analysis using capillary electrophoresis time-of-flight mass spectrometry (CE-TOF-MS). Results: One hundred and thirteen substances were identified in the saliva. Glycine was the most abundant amino acid in the saliva, followed by alanine, serine and proline. After the start of the template therapy, her headache was alleviated, accompanied by a significant (p=0.042) increase of salivary concentration of glycine, as compared with total amino acids whereas that of other amino acids was not significantly changed. In the metabolomics profile, salivary concentration of large number of metabolites as compared with total metabolite concentration decreased, including N-acetylneuraminate (p=0.025) and p-hydroxyphenylacetate (p=0.039). Conclusion: This pilot study demonstrated, to our knowledge for the first time, that only glycine exhibited unique changes among total metabolites, suggesting its significant role in template therapy.

Original languageEnglish
Pages (from-to)1015-1020
Number of pages6
JournalIn Vivo
Issue number6
Publication statusPublished - 2012


  • Metabolite profiling
  • Salivary glycine
  • Template therapy

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology
  • Cancer Research
  • Pharmacology


Dive into the research topics of 'Pilot study of changes in salivary metabolic profiles induced by template therapy'. Together they form a unique fingerprint.

Cite this