PMab-301: An Anti-Giraffe Podoplanin Monoclonal Antibody for Immunohistochemistry

Tsunenori Ouchida; Tomohiro Tanaka; Hiroyuki Suzuki; Kazuyuki Uchida; Takayuki Nakagawa; Guanjie Li; Takuro Nakamura; Miyuki Yanaka; Saori Handa; Mika K. Kaneko; Yukinari Kato

doi:10.1089/mab.2023.0020

PMab-301: An Anti-Giraffe Podoplanin Monoclonal Antibody for Immunohistochemistry

Tsunenori Ouchida
, Tomohiro Tanaka
, Hiroyuki Suzuki
, Kazuyuki Uchida
, Takayuki Nakagawa
, Guanjie Li
, Takuro Nakamura
, Miyuki Yanaka
, Saori Handa
, Mika K. Kaneko
, Yukinari Kato

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Immunohistochemistry staining is an essential method in pathological diagnoses. Podoplanin (PDPN) is a specific maker of alveolar epithelium, lymphatic vessels, and glomeruli. In this study, we established a novel anti-giraffe PDPN (girPDPN) mAb, PMab-301, using the Cell-Based Immunization and Screening (CBIS) method. PMab-301 (mouse IgG₁, kappa) detected girPDPN in various applications, such as flow cytometry, western blot, and immunohistochemistry. PMab-301 specifically stained type-I alveolar cells using formalin-fixed paraffin-embedded giraffe lung tissues. Our findings suggest the potential usefulness of PMab-301 for the pathophysiological analyses of giraffe tissues.

Original language	English
Pages (from-to)	209-215
Number of pages	7
Journal	Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
Volume	42
Issue number	6
DOIs	https://doi.org/10.1089/mab.2023.0020
Publication status	Published - 2023 Dec 1
Externally published	Yes

Keywords

giraffe
PDPN
PMab-301
podoplanin

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1089/mab.2023.0020

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title = "PMab-301: An Anti-Giraffe Podoplanin Monoclonal Antibody for Immunohistochemistry",

abstract = "Immunohistochemistry staining is an essential method in pathological diagnoses. Podoplanin (PDPN) is a specific maker of alveolar epithelium, lymphatic vessels, and glomeruli. In this study, we established a novel anti-giraffe PDPN (girPDPN) mAb, PMab-301, using the Cell-Based Immunization and Screening (CBIS) method. PMab-301 (mouse IgG1, kappa) detected girPDPN in various applications, such as flow cytometry, western blot, and immunohistochemistry. PMab-301 specifically stained type-I alveolar cells using formalin-fixed paraffin-embedded giraffe lung tissues. Our findings suggest the potential usefulness of PMab-301 for the pathophysiological analyses of giraffe tissues.",

keywords = "giraffe, PDPN, PMab-301, podoplanin",

author = "Tsunenori Ouchida and Tomohiro Tanaka and Hiroyuki Suzuki and Kazuyuki Uchida and Takayuki Nakagawa and Guanjie Li and Takuro Nakamura and Miyuki Yanaka and Saori Handa and Kaneko, \{Mika K.\} and Yukinari Kato",

year = "2023",

month = dec,

day = "1",

doi = "10.1089/mab.2023.0020",

language = "English",

volume = "42",

pages = "209--215",

journal = "Monoclonal Antibodies in Immunodiagnosis and Immunotherapy",

issn = "2167-9436",

publisher = "SAGE Publications Ltd",

number = "6",

}

TY - JOUR

T1 - PMab-301

T2 - An Anti-Giraffe Podoplanin Monoclonal Antibody for Immunohistochemistry

AU - Ouchida, Tsunenori

AU - Tanaka, Tomohiro

AU - Suzuki, Hiroyuki

AU - Uchida, Kazuyuki

AU - Nakagawa, Takayuki

AU - Li, Guanjie

AU - Nakamura, Takuro

AU - Yanaka, Miyuki

AU - Handa, Saori

AU - Kaneko, Mika K.

AU - Kato, Yukinari

PY - 2023/12/1

Y1 - 2023/12/1

N2 - Immunohistochemistry staining is an essential method in pathological diagnoses. Podoplanin (PDPN) is a specific maker of alveolar epithelium, lymphatic vessels, and glomeruli. In this study, we established a novel anti-giraffe PDPN (girPDPN) mAb, PMab-301, using the Cell-Based Immunization and Screening (CBIS) method. PMab-301 (mouse IgG1, kappa) detected girPDPN in various applications, such as flow cytometry, western blot, and immunohistochemistry. PMab-301 specifically stained type-I alveolar cells using formalin-fixed paraffin-embedded giraffe lung tissues. Our findings suggest the potential usefulness of PMab-301 for the pathophysiological analyses of giraffe tissues.

AB - Immunohistochemistry staining is an essential method in pathological diagnoses. Podoplanin (PDPN) is a specific maker of alveolar epithelium, lymphatic vessels, and glomeruli. In this study, we established a novel anti-giraffe PDPN (girPDPN) mAb, PMab-301, using the Cell-Based Immunization and Screening (CBIS) method. PMab-301 (mouse IgG1, kappa) detected girPDPN in various applications, such as flow cytometry, western blot, and immunohistochemistry. PMab-301 specifically stained type-I alveolar cells using formalin-fixed paraffin-embedded giraffe lung tissues. Our findings suggest the potential usefulness of PMab-301 for the pathophysiological analyses of giraffe tissues.

KW - giraffe

KW - PDPN

KW - PMab-301

KW - podoplanin

UR - https://www.scopus.com/pages/publications/85181396317

UR - https://www.scopus.com/pages/publications/85181396317#tab=citedBy

U2 - 10.1089/mab.2023.0020

DO - 10.1089/mab.2023.0020

M3 - Article

C2 - 38150189

AN - SCOPUS:85181396317

SN - 2167-9436

VL - 42

SP - 209

EP - 215

JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

IS - 6

ER -

PMab-301: An Anti-Giraffe Podoplanin Monoclonal Antibody for Immunohistochemistry

Abstract

Keywords

ASJC Scopus subject areas

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